Anti-Ten-1 antibody [EPR29567-15] - C-terminal

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ten-1 antibody [EPR29567-15] - C-terminal (AB327382)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A-673 (human muscle Ewings sarcoma cell line) and MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Ten-1 with ab327382 at 1/50 (9.48 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in A-673 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : MCF7.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ten-1 antibody [EPR29567-15] - C-terminal (AB327382)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCI-H69 (human lung small cell carcinoma cell line) and HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Ten-1 with ab327382 at 1/50 (9.48 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in NCI-H69 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : HeLa.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

• I-ELISA

Lab

Indirect ELISA - Anti-Ten-1 antibody [EPR29567-15] - C-terminal (AB327382)

Indirect ELISA analysis of ab327382 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Ten-1 recombinant protein; Human Ten-2 recombinant protein; Human Ten-3 recombinant protein; Human Ten-4 recombinant protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with recombinant human Ten-2, Ten-3 or Ten-4 by Elisa.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ten-1 antibody [EPR29567-15] - C-terminal (AB327382)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes labelling Ten-1 with ab327382 at 1/50 (9.48 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Low expression : Confocal image showing no staining in mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-mouse CD3 rat monoclonal antibody (Alexa Fluor® 647) was used to counterstain (Magenta).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ten-1 antibody [EPR29567-15] - C-terminal (AB327382)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling Ten-1 with ab327382 at 1/50 (9.48 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary neural/glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).