AB86182

Anti-Tenascin C antibody [DB7]

Name: Anti-Tenascin C antibody [DB7] (ab86182) | Abcam
Brand: Abcam Limited
SKU: AB86182
Price: 525 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal Tenascin C antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNC.

View Alternative Names

HXB, TNC, Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [DB7] (AB86182)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [DB7] (AB86182)

Immunohistochemical analysis of human uterus tissue labeling Tenascin C with ab98597 at 1/400 dilution.

Pretreated with enzymatic digestion 37°C for 10 minutes.

Lab

Western blot - Anti-Tenascin C antibody [DB7] (AB86182)

ab86182 was shown to react with Tenascin C in western blot. Membranes were blocked in 2 % BSA in TBS-T (0.1 % Tween^®) before incubation with ab86182 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Mouse (H+L) secondary antibody at 1 in 5000 dilution for 1 hour at room temperature before development with Optiblot ECL reagent (ab133406) and imaging.

All lanes:

Western blot - Anti-Tenascin C antibody [DB7] (ab86182) at 1/1000 dilution

Lane 1:

U-87 MG Treated BFA (5µg/mL, 6h) cell lysate at 20 µg

Lane 2:

U-87 MG cell lysate at 20 µg

Predicted band size: 241 kDa

false

Exposure time: 20s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DB7

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human TNC.

P24821

Specificity

ab86182 reacts with the fibrinogen like knob domain of Tenascin C.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab86182 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tenascin C also known as hexabrachion is an extracellular matrix glycoprotein with a mass of approximately 220-300 kDa depending on post-translational modifications. It shows high expression in areas undergoing tissue remodeling such as embryonic tissues wounds and tumors. It consists of repeating structural elements including epidermal growth factor-like repeats and fibronectin type III domains which facilitate its function in the context of tissue dynamics.

Biological function summary

The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.

Pathways

Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.

Tenascin C exhibits a strong connection to cancer progression and chronic inflammatory conditions such as rheumatoid arthritis. Its levels rise in tumors and inflamed tissues where it interacts with other matrix components and cytokines like interleukin-6 (IL-6). These interactions contribute to a microenvironment that promotes tumor growth and persistence of inflammation highlighting its role in the pathophysiology of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed : 19884327).

See full target information TNC

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 11: PubMed36294311

2022

Expression Pattern of Tenascin-C, Matrilin-2, and Aggrecan in Diseases Affecting the Corneal Endothelium.

Applications

Unspecified application

Species

Unspecified reactive species

Gréta Varkoly,Tibor G Hortobágyi,Enikő Gebri,János Bencze,Tibor Hortobágyi,László Módis

Molecular vision 27:26-36 PubMed33633437

2021

Extracellular matrix changes in corneal opacification vary depending on etiology.

Applications

Unspecified application

Species

Unspecified reactive species

László V Módis,Gréta Varkoly,János Bencze,Tibor G Hortobágyi,László Módis,Tibor Hortobágyi

Medicine 98:e14045 PubMed30633201

2019

Tenascin-C expression is significantly associated with the progression and prognosis in gastric GISTs.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyong Shen,Chengshi Wang,Yuan Yin,Huijiao Chen,Xiaonan Yin,Zhaolun Cai,Zhixin Chen,Bo Zhang,Zongguang Zhou

Microvascular research 118:101-112 PubMed29550275

2018

Generation of human umbilical cord vein CD146+ perivascular cell origined three-dimensional vascular construct.

Applications

IHC-P

Species

Unspecified reactive species

Beyza Gökçinar-Yagci,Nilgün Yersal,Petek Korkusuz,Betül Çelebi-Saltik

Oncotarget 9:1760-1771 PubMed29416729

2018

Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

David Onion,Mark Isherwood,Naveen Shridhar,Mikalena Xenophontos,Madeleine L Craze,Laura J Day,María A García-Márquez,Robert G Pineda,Alexander M Reece-Smith,John H Saunders,John P Duffy,Richard H Argent,Anna M Grabowska

BioMed research international 2017:7360354 PubMed29085840

2017

Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwan Li,Ziming Liu,Ying Jin,Xizhong Zhu,Shengmin Wang,Jibin Yang,Youliang Ren,Qiang Fu,Huazhang Xiong,Gang Zou,Yi Liu

Molecular vision 18:1927-36 PubMed22876117

2012

Fibrillin-2, tenascin-C, matrilin-2, and matrilin-4 are strongly expressed in the epithelium of human granular and lattice type I corneal dystrophies.

Applications

IHC-P

Species

Human

Eszter Szalai,Szabolcs Felszeghy,Zoltán Hegyi,László Módis,András Berta,Kai Kaarniranta

The American journal of pathology 141:907-13 PubMed1384340

1992

Enhanced tenascin expression in cervical and vulvar koilocytotic lesions.

Applications

Unspecified application

Species

Unspecified reactive species

O Tiitta,T Wahlström,J Paavonen,A Linnala,S Sharma,V E Gould,I Virtanen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tenascin C antibody [DB7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [DB7] (AB86182)

Western blot - Anti-Tenascin C antibody [DB7] (AB86182)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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