Anti-Tenascin C antibody [EB2]

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EB2] (AB88280)

ab88280, staining Tenascin C in vulva carcinoma frozen tissue sections by Immunohistochemistry.

• WB

Lab

Western blot - Anti-Tenascin C antibody [EB2] (AB88280)

False colour image of Western blot : Anti-Tenascin C antibody [EB2] staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (ab108198) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab88280 was shown to bind specifically to Tenascin C. A band was observed at 200/270 kDa in treated wild-type U-2 OS cell lysates with no signal observed at this size in TNC knockout cell line ab262488 (knockout cell lysate ab263924). To generate this image, wild-type and TNC knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 2 % BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 16 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Mouse (H+L) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Tenascin C antibody [EB2] (ab88280) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate at 40 µg

Lane 2:

TNC knockout U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate at 40 µg

Lane 2:

Western blot - Human TNC (Tenascin C) knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-tnc-tenascin-c-knockout-u-2-os-cell-line-ab262488'>ab262488</a>)

Lane 3:

Wild-type U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 40 µg

Lane 4:

TNC knockout U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 40 µg

Lane 5:

U-87 MG Treated BFA (5ug/mL, 6h) cell lysate at 40 µg

Lane 6:

U-87 MG cell lysate at 40 µg

Predicted band size: 241 kDa

Observed band size: 200 kDa,270 kDa

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