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Mouse Monoclonal Tenascin C antibody. Suitable for WB, IHC-Fr and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNC.


Images

Western blot - Anti-Tenascin C antibody [EB2] (AB88280), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EB2] (AB88280), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human TNC. Database link P24821

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-Fr
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).

Alternative names

HXB, TNC, Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C

Recommended products

Mouse Monoclonal Tenascin C antibody. Suitable for WB, IHC-Fr and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNC.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Human TNC. Database link P24821
Clone number
EB2
Purification technique
Affinity purification Protein G
Specificity

ab88280 reacts with the 4th and 5th fibronectin repeats of human
Tenascin C polypeptides.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes


ab88280 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tenascin C also known as hexabrachion is an extracellular matrix glycoprotein with a mass of approximately 220-300 kDa depending on post-translational modifications. It shows high expression in areas undergoing tissue remodeling such as embryonic tissues wounds and tumors. It consists of repeating structural elements including epidermal growth factor-like repeats and fibronectin type III domains which facilitate its function in the context of tissue dynamics.

Biological function summary

The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.

Pathways

Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.

Associated diseases and disorders

Tenascin C exhibits a strong connection to cancer progression and chronic inflammatory conditions such as rheumatoid arthritis. Its levels rise in tumors and inflamed tissues where it interacts with other matrix components and cytokines like interleukin-6 (IL-6). These interactions contribute to a microenvironment that promotes tumor growth and persistence of inflammation highlighting its role in the pathophysiology of these diseases.

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2 product images

  • Western blot - Anti-Tenascin C antibody [EB2] (ab88280), expandable thumbnail

    Western blot - Anti-Tenascin C antibody [EB2] (ab88280)

    False colour image of Western blot: Anti-Tenascin C antibody [EB2] staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (Anti-alpha Actinin 4 antibody [EPR2533(2)] ab108198) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab88280 was shown to bind specifically to Tenascin C. A band was observed at 200/270 kDa in treated wild-type U-2 OS cell lysates with no signal observed at this size in TNC knockout cell line Human TNC (Tenascin C) knockout U-2 OS cell line ab262488 (knockout cell lysate ab263924). To generate this image, wild-type and TNC knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 2 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged with 16 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Mouse (H+L) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.

    All lanes: Western blot - Anti-Tenascin C antibody [EB2] (ab88280) at 1/1000 dilution

    Lane 1: Wild-type U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate at 40 µg

    Lane 2: TNC knockout U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate at 40 µg

    Lane 2: Western blot - Human TNC (Tenascin C) knockout U-2 OS cell line (Human TNC (Tenascin C) knockout U-2 OS cell line ab262488)

    Lane 3: Wild-type U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 40 µg

    Lane 4: TNC knockout U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 40 µg

    Lane 5: U-87 MG Treated BFA (5ug/mL, 6h) cell lysate at 40 µg

    Lane 6: U-87 MG cell lysate at 40 µg

    Performed under reducing conditions.

    Predicted band size: 241 kDa

    Observed band size: 200 kDa, 270 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EB2] (ab88280), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EB2] (ab88280)

    ab88280, staining Tenascin C in vulva carcinoma frozen tissue sections by Immunohistochemistry.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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