Name: Anti-Tenascin C antibody [EPR4219]
SKU: ab108930
Rating: 4 (16 reviews)

Anti-Tenascin C antibody [EPR4219] (ab108930) is a rabbit monoclonal antibody detecting Tenascin C in Western Blot, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 90 publications

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Images

Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (AB108930), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P	IHC-Fr
Human	Not recommended	Not recommended	Tested	Expected	Expected
Mouse	Not recommended	Not recommended	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Tested	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

8 products for Alternative Product

1 product for Alternative Version

Target data

See full target information TNC

Function

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).

Alternative names

HXB, TNC, Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR4219
Purification technique: Affinity purification Protein A
Specificity: IHC on human tissues which we tested (such as testis, pancreas and stomach) showed non-specific staining. We don't recommend this antibody for IHC on human tissues.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Product Specifications
Anti-Tenascin C antibody [EPR4219] (ab108930) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-Fr, IHC-P, WB in human, mouse, rat samples.
Anti-Tenascin C antibody [EPR4219] (ab108930) specifically detects Tenascin C (UniProt ID: P24821; Molecular weight: 239kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Tenascin C antibody [EPR4219] (ab108930) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Tenascin C antibody [EPR4219] (ab108930) has been confirmed by testing in knockout samples.
Anti-Tenascin C antibody [EPR4219] (ab108930) has been cited over 95 times in peer reviewed journals and is trusted by the scientific community.
Anti-Tenascin C antibody [EPR4219] (ab108930) has 10 independent reviews from customers.

Related Products
Antibody clone EPR4219 is also available pre-conjugated to a variety of labels for your convenience - HRP, PE, APC, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 555, Alexa Fluor® 647, Alexa Fluor® 750 (ab196918, ab317894, ab317931, ab317971, ab318012, ab318054, ab318096, ab320917).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)
Immunohistochemistry (Frozen sections) analysis of mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an AlexaFluor^®488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Liver is negative control (PMID: 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution
Lane 1: Human fetal brain at 20 µg
Lane 2: Human liver at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 241 kDa, 33 kDa, 45 kDa
Observed band size: 250 kDa, 42 kDa, 45 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution
All lanes: U87-MG (human glioblastoma) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 241 kDa
Observed band size: 250 kDa
Exposure time: 3min
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)
Immunohistochemistry (Frozen sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an AlexaFluor^®488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)
Immunohistochemistry (Frozen sections) analysis of mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an AlexaFluor^®488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043) is observed.
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time:
Lane 1: 15 seconds
Lane 2: 30 seconds
The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution
Lane 1: Postnatal (P0) mouse cerebellum at 20 µg
Lane 2: Postnatal (P0) rat brain at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 241 kDa
Observed band size: 250 kDa
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)
All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution
All lanes: Human fetal brain lysate at 10 µg
Predicted band size: 241 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Hematoxylin was used to counterstain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043) is observed.
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)
Western blot: Anti-TNC antibody [EPR4219] (ab108930) staining at 1/500 dilution, shown in black; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab108930 was shown to bind specifically to TNC. A band was observed at 122 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in TNC knockout cell line. To generate this image, wild-type and TNC knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 2 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 5 seconds exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/500 dilution
Lane 1: Wild-type U-2 OS BFA (5 ug/mL, 6 h) cell lysate at 40 µg
Lane 2: Wild-type U-2 OS BFA (0 ug/mL, 6 h) cell lysate at 40 µg
Lane 3: TNC knockout U-2 OS BFA (5 ug/mL, 6 h) cell lysate at 40 µg
Lane 4: TNC knockout U-2 OS BFA (0 ug/mL, 6 h) cell lysate at 40 µg
Lane 5: U-87 MG Treated BFA (5 ug/mL, 6 h) cell lysate at 10 µg
Lane 6: U-87 MG Control BFA (0 ug/mL, 6 h) cell lysate at 10 µg
Secondary
All lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.