Skip to main content

Anti-Tenascin C antibody [EPR4219] ab108930 is a rabbit monoclonal antibody that is used in Tenascin C western blotting and IHC. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR4219 is the most widely used clone for Tenascin C on the market and is cited in >110 publications
- Specificity confirmed with TNC knockout cell line validation


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (AB108930), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (AB108930), expandable thumbnail
  • Western blot - Anti-Tenascin C antibody [EPR4219] (AB108930), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (AB108930), expandable thumbnail
  • Western blot - Anti-Tenascin C antibody [EPR4219] (AB108930), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-Fr
Human
Not recommended
Not recommended
Tested
Expected
Expected
Mouse
Not recommended
Not recommended
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Tested
Tested
Tested

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

1 product for Alternative Version

Target data

Function

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).

Alternative names

Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C, HXB, TNC

Recommended products

Anti-Tenascin C antibody [EPR4219] ab108930 is a rabbit monoclonal antibody that is used in Tenascin C western blotting and IHC. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR4219 is the most widely used clone for Tenascin C on the market and is cited in >110 publications
- Specificity confirmed with TNC knockout cell line validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4219

Purification technique

Affinity purification Protein A

Specificity

IHC on human tissues which we tested (such as testis, pancreas and stomach) showed non-specific staining. We don't recommend this antibody for IHC on human tissues.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tenascin C also known as hexabrachion is an extracellular matrix glycoprotein with a mass of approximately 220-300 kDa depending on post-translational modifications. It shows high expression in areas undergoing tissue remodeling such as embryonic tissues wounds and tumors. It consists of repeating structural elements including epidermal growth factor-like repeats and fibronectin type III domains which facilitate its function in the context of tissue dynamics.

Biological function summary

The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.

Pathways

Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.

Associated diseases and disorders

Tenascin C exhibits a strong connection to cancer progression and chronic inflammatory conditions such as rheumatoid arthritis. Its levels rise in tumors and inflamed tissues where it interacts with other matrix components and cytokines like interleukin-6 (IL-6). These interactions contribute to a microenvironment that promotes tumor growth and persistence of inflammation highlighting its role in the pathophysiology of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.

  • Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Immunohistochemistry (Frozen sections) analysis of mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

    Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.

  • Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    Liver is negative control (PMID: 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

    Lane 1: Human fetal brain at 20 µg

    Lane 2: Human liver at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 241 kDa, 33 kDa, 45 kDa

    Observed band size: 250 kDa, 42 kDa, 45 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain.

    Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.

  • Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

    All lanes: U87-MG (human glioblastoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 241 kDa

    Observed band size: 250 kDa

    Exposure time: 3min

  • Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Immunohistochemistry (Frozen sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

    Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.

  • Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Immunohistochemistry (Frozen sections) analysis of mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

    Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043) is observed.

  • Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure Time:
    Lane 1: 15 seconds
    Lane 2: 30 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

    Lane 1: Postnatal (P0) mouse cerebellum at 20 µg

    Lane 2: Postnatal (P0) rat brain at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 241 kDa

    Observed band size: 250 kDa

  • Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

    All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

    All lanes: Human fetal brain lysate at 10 µg

    Predicted band size: 241 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Hematoxylin was used to counterstain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043) is observed.

  • Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930), expandable thumbnail

    Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

    Western blot: Anti-TNC antibody [EPR4219] (ab108930) staining at 1/500 dilution, shown in black; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab108930 was shown to bind specifically to TNC. A band was observed at 122 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in TNC knockout cell line. To generate this image, wild-type and TNC knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 2 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 5 seconds exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/500 dilution

    Lane 1: Wild-type U-2 OS BFA (5 ug/mL, 6 h) cell lysate at 40 µg

    Lane 2: Wild-type U-2 OS BFA (0 ug/mL, 6 h) cell lysate at 40 µg

    Lane 3: TNC knockout U-2 OS BFA (5 ug/mL, 6 h) cell lysate at 40 µg

    Lane 4: TNC knockout U-2 OS BFA (0 ug/mL, 6 h) cell lysate at 40 µg

    Lane 5: U-87 MG Treated BFA (5 ug/mL, 6 h) cell lysate at 10 µg

    Lane 6: U-87 MG Control BFA (0 ug/mL, 6 h) cell lysate at 10 µg

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com