AB3970

Anti-Tenascin C antibody [T2H5]

Name: Anti-Tenascin C antibody [T2H5] Mouse monoclonal (ab3970) | Abcam
Brand: Abcam Limited
SKU: AB3970
Price: 435 USD
Availability: InStock

(1 Review)

(24 Publications)

Mouse Monoclonal Tenascin C antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 24 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNC.

View Alternative Names

HXB, TNC, Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C

2 Images

Supplier Data

Western blot - Anti-Tenascin C antibody [T2H5] (AB3970)

All lanes:

Western blot - Anti-Tenascin C antibody [T2H5] (ab3970) at 2 µg/mL

Lane 1:

U87-MG cell lysates, non-reduced

Lane 2:

Jurkat cell lysates, non-reduced

Lane 3:

U87-MG cell lysates, reduced

Lane 4:

Jurkat cell lysates, reduced

Secondary

All lanes:

IRDye800-conjugated anti-mouse secondary antibody

Predicted band size: 241 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [T2H5] (AB3970)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [T2H5] (AB3970)

ab3970 staining paraffin embedded tonsil tissue sections.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

T2H5

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Human TNC.

P24821

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

Storage buffer

pH: 8 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tenascin C also known as hexabrachion is an extracellular matrix glycoprotein with a mass of approximately 220-300 kDa depending on post-translational modifications. It shows high expression in areas undergoing tissue remodeling such as embryonic tissues wounds and tumors. It consists of repeating structural elements including epidermal growth factor-like repeats and fibronectin type III domains which facilitate its function in the context of tissue dynamics.

Biological function summary

The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.

Pathways

Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.

Tenascin C exhibits a strong connection to cancer progression and chronic inflammatory conditions such as rheumatoid arthritis. Its levels rise in tumors and inflamed tissues where it interacts with other matrix components and cytokines like interleukin-6 (IL-6). These interactions contribute to a microenvironment that promotes tumor growth and persistence of inflammation highlighting its role in the pathophysiology of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed : 19884327).

See full target information TNC

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40387093

2025

Human CD29+/CD56+ myogenic progenitors display tenogenic differentiation potential and facilitate tendon regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiexiang Shao,Xingzuan Lin,Hao Zhou,Minghui Wang,Lili Han,Xin Fu,Sheng Li,Siyuan Zhu,Shenao Zhou,Wenjun Yang,Jianhua Wang,Zhanghua Li,Ping Hu

Scientific reports 15:14654 PubMed40287463

2025

Comprehensive identification of dysregulated extracellular matrix molecules in the corneal endothelium of patients with Fuchs endothelial corneal dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Soichiro Inagaki,Hanielle Vaitinadapoule,Taichi Yuasa,Tatsuya Nakagawa,Masaya Ikegawa,Yumiko Toyama,Takashi Nirasawa,Yuichi Tokuda,Masakazu Nakano,Kei Tashiro,Theofilos Tourtas,Ursula Schlötzer-Schrehardt,Friedrich Kruse,Ines Aouimeur,Zhiguo He,Philippe Gain,Noriko Koizumi,Gilles Thuret,Naoki Okumura

Endocrinology 166: PubMed39951495

2025

Tenascin-C Potentiates Wnt Signaling in Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Heather A Hartmann,Matthew A Loberg,George J Xu,Anna C Schwarzkopf,Sheau-Chiann Chen,Courtney J Phifer,Kailey Caroland,Hua-Chang Chen,Diana Diaz,Megan L Tigue,Amanda B Hesterberg,Jean-Nicolas Gallant,Sophia M Shaddy,Quanhu Sheng,James L Netterville,Sarah L Rohde,Carmen C Solórzano,Lindsay A Bischoff,Naira Baregamian,Paula J Hurley,Barbara A Murphy,Jennifer H Choe,Eric C Huang,Fei Ye,Ethan Lee,Vivian L Weiss

PNAS nexus 3:pgae355 PubMed39285933

2024

Collagen VI deposition primes the glioblastoma microenvironment for invasion through mechanostimulation of β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Junghwa Cha,Erika A Ding,Emily M Carvalho,Annabelle Fowler,Manish K Aghi,Sanjay Kumar

Scientific reports 13:10401 PubMed37369713

2023

Shotgun proteomics identification of proteins expressed in the Descemet's membrane of patients with Fuchs endothelial corneal dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Nakagawa,Naoki Okumura,Masaya Ikegawa,Yumiko Toyama,Takashi Nirasawa,Frederic Mascarelli,Hanielle Vaitinadapoule,Ines Aouimeur,Zhiguo He,Philippe Gain,Gilles Thuret,Noriko Koizumi

Biomaterials 286:121606 PubMed35660820

2022

Mechanical activation drives tenogenic differentiation of human mesenchymal stem cells in aligned dense collagen hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeree Park,Showan N Nazhat,Derek H Rosenzweig

Scientific reports 12:4462 PubMed35292718

2022

The possible role of hypoxia in the affected tissue of relapsed clubfoot.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Novotny,Adam Eckhardt,Martina Doubkova,Jarmila Knitlova,David Vondrasek,Eliska Vanaskova,Martin Ostadal,Jiri Uhlik,Lucie Bacakova,Jana Musilkova

Biomaterials 279:121204 PubMed34736146

2021

Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Helen Edwards,Gemma Louise Jones,Anthony Herbert,John Fisher,Eileen Ingham

Cell research : PubMed32839552

2020

Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shen'ao Zhou,Wei Zhang,Gaihong Cai,Yingzhe Ding,Caixia Wei,Sheng Li,Yu Yang,Jie Qin,Dan Liu,Hao Zhang,Xiexiang Shao,Jianhua Wang,Hongye Wang,Wenjun Yang,Huating Wang,She Chen,Ping Hu,Liming Sun

JCI insight 5: PubMed32045383

2020

Fibroblast subtypes define a metastatic matrisome in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Heather M Brechbuhl,Alexander S Barrett,Etana Kopin,Jaime C Hagen,Amy L Han,Austin E Gillen,Jessica Finlay-Schultz,Diana M Cittelly,Philip Owens,Kathryn B Horwitz,Carol A Sartorius,Kirk Hansen,Peter Kabos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tenascin C antibody [T2H5]

Western blot - Anti-Tenascin C antibody [T2H5] (AB3970)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [T2H5] (AB3970)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

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