Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (AB325134)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Teneurin-2 with ab325134 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (AB325134)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Teneurin-2 with ab325134 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse hippocampus.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (AB325134)

Immunohistochemical analysis of paraffin-embedded Mouse whole brain tissue labeling Teneurin-2 with ab325134 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse whole brain.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (AB325134)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : THP-1, HeLa.

The band pattern observed is consistent with what has been described in the literature (PMID : 27499734).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane 1 : 180 seconds; Lanes 2-6 : 3 seconds.

All lanes:

Western blot - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (ab325134) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

TT (human thyroid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 290 kDa,270 kDa,200 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (AB325134)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, skeletal muscle, lung, testis (PMID : 10574461).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (ab325134) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse E14 brain tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Mouse lung tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 290 kDa,270 kDa,200 kDa,124 kDa

false

Exposure time: 15s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (AB325134)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Teneurin-2 with ab325134 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse spleen.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (AB325134)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human Teneurin-1, Teneurin-3 or Teneurin-4 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-LIF antibody [EPR21713-25] - (ab244795) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Teneurin-2 (C-terminal) antibody [EPR29568-575] (ab325134) at 1/1000 dilution

Lane 1:

LIF-tagged human Teneurin-1 fragment at 10 ng

Lane 2:

LIF-tagged human Teneurin-2 fragment at 10 ng

Lane 3:

His-tagged human Teneurin-3 fragment at 10 ng

Lane 4:

LIF-tagged human Teneurin-4 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 46 kDa,60 kDa,45 kDa

false

Exposure time: 103s