Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Teneurin-4 with ab325755 at 1/100 (5.11 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebrum. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SR(human pleural effusion lymphoblast);HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Teneurin-4 with ab325755 at 1/50 (10.22 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in SR cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Low expression : HeLa. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab325755 at 1/50 dilution, followed by ab150120 at 1/1000 dilution.-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Teneurin-4 with ab325755 at 1/100 (5.11 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human astrocytoma. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunohistochemical analysis of paraffin-embedded Human corpus striatum tissue labeling Teneurin-4 with ab325755 at 1/100 (5.11 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human corpus striatum. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Teneurin-4 with ab325755 at 1/100 (5.11 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : RAW 264.7, mIMCD3, HeLa, HepG2.

Lanes 4-6 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (ab325755) at 1/1000 dilution

Lane 1:

L-929 (mouse connective tissue fibroblast) whole cell lysate at 40 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 40 µg

Lane 3:

mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole cell lysate at 40 µg

Lane 4:

SR (human pleural effusion lymphoblast) whole cell lysate at 40 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 6:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 307 kDa,124 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung, skeletal muscle.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (ab325755) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 40 µg

Lane 2:

Mouse cerebral cortex tissue lysate at 40 µg

Lane 3:

Mouse hippocampus tissue lysate at 40 µg

Lane 4:

Mouse spleen tissue lysate at 40 µg

Lane 5:

Mouse lung tissue lysate at 40 µg

Lane 6:

Mouse skeletal muscle tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 307 kDa,124 kDa

false

Exposure time: 180s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Teneurin-4 with ab325755 at 1/100 (5.11 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on mouse liver. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Teneurin-4 with ab325755 at 1/100 (5.11 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human liver. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Teneurin-4 with ab325755 at 1/100 (5.11 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human spleen. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• I-ELISA

Lab

Indirect ELISA - Anti-Teneurin-4 antibody [EPR29569-515] - N-terminal (AB325755)

Indirect ELISA analysis of ab325755 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human TENM1 protein;Human TENM2 protein;Human TENM3 protein;Human TENM4 protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human TENM1, human TENM2 and human TENM3.