Anti-Teneurin-4 antibody [EPR29569-564] - C-terminal

• WB

Lab

Western blot - Anti-Teneurin-4 antibody [EPR29569-564] - C-terminal (AB325683)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : RAW 264.7, mIMCD3, HeLa, HepG2.

The bands beneath the target band (260 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-Teneurin-4 antibody [EPR29569-564] - C-terminal (ab325683) at 1/1000 dilution

Lane 1:

L-929 (mouse connective tissue fibroblast) whole cell lysate at 40 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 40 µg

Lane 3:

mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole cell lysate at 40 µg

Lane 4:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 40 µg

Lane 5:

SR (human pleural effusion lymphoblast) whole cell lysate at 40 µg

Lane 6:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 7:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 307 kDa,124 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-Teneurin-4 antibody [EPR29569-564] - C-terminal (AB325683)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung, skeletal muscle.

The band beneath the target band (307 kDa) is likely to be cleaved form (PMID : 30618567).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-Teneurin-4 antibody [EPR29569-564] - C-terminal (ab325683) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 40 µg

Lane 2:

Mouse cerebral cortex tissue lysate at 40 µg

Lane 3:

Mouse hippocampus tissue lysate at 40 µg

Lane 4:

Mouse spleen tissue lysate at 40 µg

Lane 5:

Mouse lung tissue lysate at 40 µg

Lane 6:

Mouse skeletal muscle tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 260-307 kDa,124 kDa

false

Exposure time: 180s

• I-ELISA

Lab

Indirect ELISA - Anti-Teneurin-4 antibody [EPR29569-564] - C-terminal (AB325683)

Indirect ELISA analysis of ab325683 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human TENM1 protein;Human TENM2 protein;Human TENM3 protein;Human TENM4 protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human TENM1, human TENM2 and human TENM3.