Rabbit Polyclonal Tensin 3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human TNS3.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TNS3. The exact immunogen used to generate this antibody is proprietary information. Database link Q68CZ2
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| African green monkey | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
May act as a protein phosphatase and/or a lipid phosphatase (Probable). Involved in the dissociation of the integrin-tensin-actin complex (PubMed:17643115). EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1 (PubMed:17643115). Increases DOCK5 guanine nucleotide exchange activity towards Rac and plays a role in osteoclast podosome organization (By similarity). Enhances RHOA activation in the presence of DLC1 (PubMed:26427649). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces TNS3 phosphorylation which changes its binding preference from DLC1 to the p85 regulatory subunit of the PI3K kinase complex, displacing PI3K inhibitor PTEN and resulting in translocation of the TNS3-p85 complex to the leading edge of migrating cells to promote RAC1 activation (PubMed:26166433). Meanwhile, PTEN switches binding preference from p85 to DLC1 and the PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA (PubMed:26166433). Acts as an adapter protein by bridging the association of scaffolding protein PEAK1 with integrins ITGB1, ITGB3 and ITGB5 which contributes to the promotion of cell migration (PubMed:35687021). Controls tonsil-derived mesenchymal stem cell proliferation and differentiation by regulating the activity of integrin ITGB1 (PubMed:31905841).
Alternative names
TEM6, TENS1, TPP, TNS3, Tensin-3, Tensin-like SH2 domain-containing protein 1, Tumor endothelial marker 6
Recommended products
Rabbit Polyclonal Tensin 3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human TNS3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TNS3. The exact immunogen used to generate this antibody is proprietary information. Database link Q68CZ2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tensin 3 also known as TNS3 is a cytoskeletal protein with a mass of approximately 150 kDa. It functions mechanically as part of the actin cytoskeleton network. The protein is involved in linking actin filaments to integrin receptors at focal adhesions playing a significant role in the dynamics and stability of cell movement. Tensin 3 is expressed in a variety of tissues including the lung liver and intestine indicating its broad functional relevance across different cell types.
- Biological function summary
This protein contributes to the regulation of cell adhesion migration and organization. Tensin 3 often associates in complexes with other tensin family members such as Tensin 1 and Tensin 2 to perform its functions. These complexes help in the remodeling of the extracellular matrix and influence cell shape. Through interactions with these proteins Tensin 3 regulates signals that are essential for the maintenance of tissue architecture and integrity.
- Pathways
Tensin 3 plays an important part in integrin signaling and cell motility pathways. It interacts closely with proteins involved in these pathways such as vinculin and talin. The integrin-mediated pathways where Tensin 3 is active are critical for processes like cell migration and wound healing. Furthermore through these pathways Tensin 3 connects cellular signaling cascades to mechanical forces within cells facilitating proper cellular response to environmental changes.
- Associated diseases and disorders
Involvement of Tensin 3 is significant in certain cancers like lung cancer and fibrotic diseases. Overexpression of Tensin 3 has been linked to cancer cell invasion and metastasis. In fibrotic conditions its dysregulation affects normal tissue repair often leading to excessive scarring. In both contexts Tensin 3 interacts with signaling proteins like focal adhesion kinase (FAK) which further amplifies its effects on disease progression and tissue pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tensin 3 antibody (ab178554)
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Tensin 3 with ab178554 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunizing peptide.
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