Rabbit Polyclonal TET1 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human TET1.
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Publications
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- International journal of molecular sciences 24:2023Endometriosis of the Cervix: A Rare Clinical Case with the Possibility of Comparing the Eutopic and Ectopic Endometrium at the Cellular Level.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKonstantin A Toniyan,Elena Yu Gorbacheva,Valery V Boyarintsev,Irina V OgnevaPubMed 36768508
- Molecular pain 18:174480692211436712022Increased ten-eleven translocation methylcytosine dioxygenase one in dorsal root ganglion contributes to inflammatory pain in CFA rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Liu,Ling Zhang,Zhen-Hua Xu,Jie Zhu,Jia-Ling Ma,Yan-Ping Gao,Guang-Yin XuPubMed 36411533
- Neural regeneration research 18:863-8682022DNA hypomethylation promotes learning and memory recovery in a rat model of cerebral ischemia/reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang Shi,Juan Feng,Ling-Yan Jian,Xin-Yu FanPubMed 36204855
- Frontiers in oncology 12:9575112022MiR-27a-3p binds to TET1 mediated DNA demethylation of ADCY6 regulates breast cancer progression epithelial-mesenchymal transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Wu,Juanjuan Qiu,Zhenru Wu,Tao He,Chen Zhou,Qing LvPubMed 35978806
- Cell 185:3169-3185.e202022The intrinsic and extrinsic effects of TET proteins during gastrulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaifeng Cheng,Markus Mittnenzweig,Yoav Mayshar,Aviezer Lifshitz,Marko Dunjić,Yoach Rais,Raz Ben-Yair,Stephanie Gehrs,Elad Chomsky,Zohar Mukamel,Hernan Rubinstein,Katharina Schlereth,Netta Reines,Ayelet-Hashahar Orenbuch,Amos Tanay,Yonatan StelzerPubMed 35908548
- Brain pathology (Zurich, Switzerland) 32:e130802022Deficiency of Tet3 in nucleus accumbens enhances fear generalization and anxiety-like behaviors in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBu-Fang Fan,Bo Hao,Yun-Da Dai,Li Xue,Yan-Wei Shi,Lu Liu,Shou-Min Xuan,Ning Yang,Xiao-Guang Wang,Hu ZhaoPubMed 35612904
- Neuroscience bulletin 38:887-9002022Homocysteine-Induced Disturbances in DNA Methylation Contribute to Development of Stress-Associated Cognitive Decline in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi-Da Wang,Xue Wang,Yun Zhao,Bing-Hua Xue,Xiao-Tian Wang,Yu-Xin Chen,Zi-Qian Zhang,Ying-Rui Tian,Fang Xie,Ling-Jia QianPubMed 35435568
- Bioengineered 13:9369-93862022Dexmedetomidine prevents cardiomyocytes from hypoxia/reoxygenation injury via modulating tetmethylcytosine dioxygenase 1-mediated DNA demethylation of Sirtuin1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Wang,Shaowei Wang,Tong Jia,Xiaojia Sun,Zhen Xing,Hui Liu,Jie Yao,Yanlin ChenPubMed 35387565
- Theranostics 12:3007-30232022Time-restricted feeding modulates the DNA methylation landscape, attenuates hallmark neuropathology and cognitive impairment in a mouse model of vascular dementia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharmelee Selvaraji,Motakis Efthymios,Roger Sik Yin Foo,David Y Fann,Mitchell Kim Peng Lai,Christopher Li Hsian Chen,Kah Leong Lim,Thiruma V ArumugamPubMed 35547760
- Bioengineered 13:6638-66492022A novel epigenetic marker, Ten-eleven translocation family member 2 (TET2), is identified in the intractable epileptic brain and regulates ATP binding cassette subfamily B member 1 (ABCB1) in the blood-brain barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan-Cheng Kong,Li-Qin Lang,Jie Hu,Xia-Ling Zhang,Ming-Kang Zhong,Chun-Lai MaPubMed 35235761
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TET1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NFU7
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Recommend for human and mouse. Please see image legends for more protocol details. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1 µg/mL | Notes Recommend for human and mouse. Please see image legends for more protocol details. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Recommend for human and mouse and rat. Please see image legend for details of the Western blot protocol. Blocking in milk and incubation of ab191698 for 1 hour at room temperature. |
Species Rat | Dilution info 1 µg/mL | Notes Recommend for human and mouse and rat. Please see image legend for details of the Western blot protocol. Blocking in milk and incubation of ab191698 for 1 hour at room temperature. |
Species Human | Dilution info 1 µg/mL | Notes Recommend for human and mouse and rat. Please see image legend for details of the Western blot protocol. Blocking in milk and incubation of ab191698 for 1 hour at room temperature. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 20 µg/mL | Notes Recommend for human and mouse. Please see image legends for more protocol details. |
Species Human | Dilution info 20 µg/mL | Notes Recommend for human and mouse. Please see image legends for more protocol details. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Dioxygenase that plays a key role in active DNA demethylation, by catalyzing the sequential oxidation of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) (PubMed:19372391, PubMed:21496894, PubMed:21778364, PubMed:35798741). In addition to its role in DNA demethylation, plays a more general role in chromatin regulation by recruiting histone modifying protein complexes to alter histone marks and chromatin accessibility, leading to both activation and repression of gene expression (PubMed:33833093). Plays therefore a role in many biological processes, including stem cell maintenance, T- and B-cell development, inflammation regulation, genomic imprinting, neural activity or DNA repair (PubMed:31278917). Involved in the balance between pluripotency and lineage commitment of cells and plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Together with QSER1, plays an essential role in the protection and maintenance of transcriptional and developmental programs to inhibit the binding of DNMT3A/3B and therefore de novo methylation (PubMed:33833093). May play a role in pancreatic beta-cell specification during development. In this context, may function as an upstream epigenetic regulator of PAX4 presumably through direct recruitment by FOXA2 to a PAX4 enhancer to preserve its unmethylated status, thereby potentiating PAX4 expression to adopt beta-cell fate during endocrine lineage commitment (PubMed:35798741). Under DNA hypomethylation conditions, such as in female meiotic germ cells, may induce epigenetic reprogramming of pericentromeric heterochromatin (PCH), the constitutive heterochromatin of pericentromeric regions. PCH forms chromocenters in the interphase nucleus and chromocenters cluster at the prophase of meiosis. In this context, may also be essential for chromocenter clustering in a catalytic activity-independent manner, possibly through the recruitment polycomb repressive complex 1 (PRC1) to the chromocenters (By similarity). During embryonic development, may be required for normal meiotic progression in oocytes and meiotic gene activation (By similarity). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034). Isoform 1. Dioxygenase that plays a key role in active DNA demethylation (PubMed:28531272). Binds to promoters, particularly to those with high CG content (By similarity). In hippocampal neurons, isoform 1 regulates the expression of a unique subset of genes compared to isoform 2, although some overlap exists between both isoforms, hence differentially regulates excitatory synaptic transmission (By similarity). In hippocampal neuron cell cultures, isoform 1 controls both miniature excitatory postsynaptic current amplitude and frequency (By similarity). Isoform 1 may regulate genes involved in hippocampal-dependent memory, leading to positive regulation of memory, contrary to isoform 2 that may decrease memory (By similarity). Isoform 2. Dioxygenase that plays a key role in active DNA demethylation (PubMed:28531272). As isoform 1, binds to promoters, particularly to those with high CG content, however displays reduced global chromatin affinity compared with isoform 1, leading to decreased global DNA demethylation compared with isoform 1 (By similarity). Contrary to isoform 1, isoform 2 localizes during S phase to sites of ongoing DNA replication in heterochromatin, causing a significant de novo 5hmC formation, globally, and more so in heterochromatin, including LINE 1 interspersed DNA repeats leading to their activation (By similarity). In hippocampal neurons, isoform 2 regulates the expression of a unique subset of genes compared to isoform 1, although some overlap between both isoforms, hence differentially regulates excitatory synaptic transmission (By similarity). In hippocampal neuron cell cultures, isoform 2 controls miniature excitatory postsynaptic current frequency, but not amplitude (By similarity). Isoform 2 may regulate genes involved in hippocampal-dependent memory, leading to negative regulation of memory, contrary to isoform 1 that may improve memory (By similarity). In immature and partially differentiated gonadotrope cells, directly represses luteinizing hormone gene LHB expression and does not catalyze 5hmC at the gene promoter (By similarity).
Alternative names
CXXC6, KIAA1676, LCX, TET1, Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6, Leukemia-associated protein with a CXXC domain, Ten-eleven translocation 1 gene protein
Recommended products
Rabbit Polyclonal TET1 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human TET1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TET1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NFU7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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11 product images
Western blot - Anti-TET1 antibody - C-terminal (ab191698)
8% SDS gel
Running condition: 130V, 1.5 hours.
Transfer condition: Wet, 250mA, 2 hrs (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight.
Primary antibody incubation: room temperature for 1 hour in 5% NFDM/TBST.
Secondary antibody incubation: room temperature for 1 hour.
Washing condition: 5 mL TBST, 4 x 5 minutes.
All lanes: Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL
Lane 1: Mouse testis at 15 µg
Lane 2: Rat testis at 15 µg
Lane 3: Human testis at 15 µg
Lane 4: Human bladder at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 235 kDa
Observed band size: 55-72 kDa
Exposure time: 1min
Western blot - Anti-TET1 antibody - C-terminal (ab191698)
Western blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.
Running condition: 8% gel, 130V for 1.5 hrs.
Transfer condition: Wet, 250mA, 2 hrs (NC membrane).
Blocking condition: 5% non-fat milk in TBS at 4°C overnight.
Washing condition: 5ml TBST, 4 x 5 min.
Exposure system: GE ECL 1 min.
All lanes: Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL
Lane 1: K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 15 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 15 µg
SecondaryAll lanes: abbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 235 kDa
Observed band size: 235 kDa
Western blot - Anti-TET1 antibody - C-terminal (ab191698)
Western blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.
Running condition: 8% gel, 130V for 1.5 hrs.
Transfer condition: Wet, 250mA, 2 hrs (NC membrane).
Blocking condition: 5% non-fat milk in TBS at 4°C overnight.
Washing condition: 5ml TBST, 4 x 5 min.
Exposure system: GE ECL 1 min.
All lanes: Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL
Lane 1: Mouse kidney at 15 µg
Lane 2: Mouse thymus at 15 µg
Lane 3: Mouse stomach at 15 µg
SecondaryAll lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 235 kDa
Observed band size: 235 kDa
Western blot - Anti-TET1 antibody - C-terminal (ab191698)
Western blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.
Running condition: 8% gel, 130V for 1.5 hrs.
Transfer condition: Wet, 250mA, 2 hrs (NC membrane).
Blocking condition: 5% non-fat milk in TBS at 4°C overnight.
Washing condition: 5ml TBST, 4 x 5 min.
Exposure system: GE ECL 10 sec.
All lanes: Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL
Lane 1: Rat kidney at 15 µg
Lane 2: Rat thymus at 15 µg
SecondaryAll lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 235 kDa
Observed band size: 235 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (ab191698)
Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) HeLa cells labeling TET1 with ab191698 at 20 μg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 3 x 3 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (ab191698)
Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) EL4 cells (mouse T-lymphocyte cell line) labeling TET1 with ab191698 at 20 μg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on EL4 cell line. The nuclear counter stain is DAPI (blue).
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 3 x 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on human testis is observed. DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, Room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, Room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, Room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on mouse testis is observed. DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
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