Anti-TET1 antibody - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (AB191698)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.

Antigen Retrieval : Citrate buffer, pH 6.0, microwave, 20 minutes.

Endogenous peroxidase blocking : 3% H₂O₂, Room temperature, 10 minutes.

Blocking condition : 5% BSA, Room temperature, 1 hour.

Washing condition : PBS, 2 X 5 minutes.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (AB191698)

Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) HeLa cells labeling TET1 with ab191698 at 20 μg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Blocking condition : 5% BSA, room temperature, 1 hour.

Washing condition : PBS, 3 x 3 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (AB191698)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on human testis is observed. DAB staining.

Antigen Retrieval : Citrate buffer, pH 6.0, microwave, 20 minutes.

Endogenous peroxidase blocking : 3% H₂O₂, Room temperature, 10 minutes.

Blocking condition : 5% BSA, room temperature, 1 hour.

Washing condition : PBS, 2 X 5 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (AB191698)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on mouse testis is observed. DAB staining.

Antigen Retrieval : Citrate buffer, pH 6.0, microwave, 20 minutes.

Endogenous peroxidase blocking : 3% H₂O₂, Room temperature, 10 minutes.

Blocking condition : 5% BSA, room temperature, 1 hour.

Washing condition : PBS, 2 X 5 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (AB191698)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.

Antigen Retrieval : Citrate buffer, pH 6.0, microwave, 20 minutes.

Endogenous peroxidase blocking : 3% H₂O₂, Room temperature, 10 minutes.

Blocking condition : 5% BSA, Room temperature, 1 hour.

Washing condition : PBS, 2 X 5 minutes.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (AB191698)

Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) EL4 cells (mouse T-lymphocyte cell line) labeling TET1 with ab191698 at 20 μg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on EL4 cell line. The nuclear counter stain is DAPI (blue).

Blocking condition : 5% BSA, room temperature, 1 hour.

Washing condition : PBS, 3 x 3 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (AB191698)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling TET1 with ab191698 at 1 μg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.

Antigen Retrieval : Citrate buffer, pH 6.0, microwave, 20 minutes.

Endogenous peroxidase blocking : 3% H₂O₂, Room temperature, 10 minutes.

Blocking condition : 5% BSA, Room temperature, 1 hour.

Washing condition : PBS, 2 X 5 minutes.

• WB

Supplier Data

Western blot - Anti-TET1 antibody - C-terminal (AB191698)

8% SDS gel

Running condition : 130V, 1.5 hours.

Transfer condition : Wet, 250mA, 2 hrs (Nitrocellulose membrane)

Blocking condition : 5% non-fat milk in TBS at 4 overnight.

Primary antibody incubation : room temperature for 1 hour in 5% NFDM/TBST.

Secondary antibody incubation : room temperature for 1 hour.

Washing condition : 5 mL TBST, 4 x 5 minutes.

All lanes:

Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL

Lane 1:

Mouse testis at 15 µg

Lane 2:

Rat testis at 15 µg

Lane 3:

Human testis at 15 µg

Lane 4:

Human bladder at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 235 kDa

Observed band size: 55-72 kDa

true

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-TET1 antibody - C-terminal (AB191698)

Western blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.

Running condition : 8% gel, 130V for 1.5 hrs.

Transfer condition : Wet, 250mA, 2 hrs (NC membrane).

Blocking condition : 5% non-fat milk in TBS at 4°C overnight.

Washing condition : 5ml TBST, 4 x 5 min.

Exposure system : GE ECL 1 min.

All lanes:

Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL

Lane 1:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 15 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 15 µg

Secondary

All lanes:

abbit IgG antibody (HRP) at 1/10000 dilution

Predicted band size: 235 kDa

Observed band size: 235 kDa

false

• WB

Supplier Data

Western blot - Anti-TET1 antibody - C-terminal (AB191698)

Western blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.

Running condition : 8% gel, 130V for 1.5 hrs.

Transfer condition : Wet, 250mA, 2 hrs (NC membrane).

Blocking condition : 5% non-fat milk in TBS at 4°C overnight.

Washing condition : 5ml TBST, 4 x 5 min.

Exposure system : GE ECL 10 sec.

All lanes:

Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL

Lane 1:

Rat kidney at 15 µg

Lane 2:

Rat thymus at 15 µg

Secondary

All lanes:

Rabbit IgG antibody (HRP) at 1/10000 dilution

Predicted band size: 235 kDa

Observed band size: 235 kDa

false

• WB

Supplier Data

Western blot - Anti-TET1 antibody - C-terminal (AB191698)

Western blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.

Running condition : 8% gel, 130V for 1.5 hrs.

Transfer condition : Wet, 250mA, 2 hrs (NC membrane).

Blocking condition : 5% non-fat milk in TBS at 4°C overnight.

Washing condition : 5ml TBST, 4 x 5 min.

Exposure system : GE ECL 1 min.

All lanes:

Western blot - Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/mL

Lane 1:

Mouse kidney at 15 µg

Lane 2:

Mouse thymus at 15 µg

Lane 3:

Mouse stomach at 15 µg

Secondary

All lanes:

Rabbit IgG antibody (HRP) at 1/10000 dilution

Predicted band size: 235 kDa

Observed band size: 235 kDa

false