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AB325925

Anti-TET1 antibody [EPR30558-579]

  • Advanced Validation
  • RabMAb
  • Recombinant
  • 20ul selling size
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Rabbit Recombinant Monoclonal TET1 antibody. Suitable for ChIC/CUT&RUN, ChIP-seq, I-ELISA, WB and reacts with Mouse, Recombinant full length protein - Mouse samples.

View Alternative Names

Cxxc6, Kiaa1676, Tet1, Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog

9 Images
ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)

Chromatin was prepared from ES-D3 [D3] cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab325925 [EPR30558-579]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)

Chromatin was prepared from ES-D3 [D3] cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab325925 [EPR30558-579]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)

Chromatin was prepared from ES-D3 [D3] cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab325925 [EPR30558-579]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

Western blot - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • WB

Lab

Western blot - Anti-TET1 antibody [EPR30558-579] (AB325925)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-TET1 antibody [EPR30558-579] (ab325925) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

F9 transfected with siRNA specifically targeting TET1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • WB

Lab

Western blot - Anti-TET1 antibody [EPR30558-579] (AB325925)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : A20.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-TET1 antibody [EPR30558-579] (ab325925) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • WB

Lab

Western blot - Anti-TET1 antibody [EPR30558-579] (AB325925)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-TET1 antibody [EPR30558-579] (ab325925) at 1/1000 dilution

All lanes:

ES-D3 [D3]  (mouse blastocyst-derived embryonic stem cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa

false

Exposure time: 180s

ChIC/CUT&RUN sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab325925 [EPR30558-579]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-TET1 antibody [EPR30558-579] (AB325925)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab325925 [EPR30558-579]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Indirect ELISA - Anti-TET1 antibody [EPR30558-579] (AB325925)
  • I-ELISA

Lab

Indirect ELISA - Anti-TET1 antibody [EPR30558-579] (AB325925)

Indirect ELISA analysis of ab325925 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Mouse TET1; Mouse TET2; Mouse TET3.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with Mouse TET2 and Mouse TET3 by ELISA.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30558-579

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ChIP-seq, ChIC/CUT&RUN, I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUN" : {"fullname" : "ChIC/CUT&RUN", "shortname":"ChIC/CUT&RUN"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ChICCUTRUN-species-checked": "testedAndGuaranteed", "ChICCUTRUN-species-dilution-info": "5 µg", "ChICCUTRUN-species-notes": "<p></p>", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "4 µg", "ChIPseq-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "ChICCUTRUN-species-checked": "notRecommended", "ChICCUTRUN-species-dilution-info": "", "ChICCUTRUN-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Mouse": { "ChICCUTRUN-species-checked": "notRecommended", "ChICCUTRUN-species-dilution-info": "", "ChICCUTRUN-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "31.25 ng/mL", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dioxygenase that plays a key role in active DNA demethylation, by catalyzing the sequential oxidation of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) (PubMed : 20639862, PubMed : 21496894, PubMed : 21778364). In addition to its role in DNA demethylation, plays a more general role in chromatin regulation by recruiting histone modifying protein complexes to alter histone marks and chromatin accessibility, leading to both activation and repression of gene expression (PubMed : 28504700, PubMed : 32286661). Plays therefore a role in many biological processes, including stem cell maintenance, T- and B-cell development, inflammation regulation, iron homeostasis, neural activity or DNA repair (PubMed : 20639862, PubMed : 31089182, PubMed : 32855402, PubMed : 33895792). Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification (PubMed : 20639862, PubMed : 28504700). Together with QSER1, plays an essential role in the protection and maintenance of transcriptional and developmental programs to inhibit the binding of DNMT3A/3B and therefore de novo methylation (By similarity). May play a role in the pancreatic beta-cell specification during development. In this context, may function as an upstream epigenetic regulator of PAX4 presumably through direct recruitment by FOXA2 to a PAX4 enhancer to preserve its unmethylated status, thereby potentiating PAX4 expression to adopt beta-cell fate during endocrine lineage commitment (By similarity). Under DNA hypomethylation conditions, such as in female meiotic germ cells, may induce epigenetic reprogramming of pericentromeric heterochromatin (PCH), the constitutive heterochromatin of pericentromeric regions. PCH forms chromocenters in the interphase nucleus and chromocenters cluster at the prophase of meiosis. In this context, may also be essential for chromocenter clustering in a catalytic activity-independent manner, possibly through the recruitment polycomb repressive complex 1 (PRC1) to the chromocenters (PubMed : 34166371). During embryonic development, may be required for normal meiotic progression in oocytes and meiotic gene activation (PubMed : 23151479). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (By similarity).. Isoform 1. Dioxygenase that plays a key role in active DNA demethylation (PubMed : 27916660, PubMed : 36056023). Binds to promoters, particularly to those with high CG content (PubMed : 27916660). In hippocampal neurons, isoform 1 regulates the expression of a unique subset of genes compared to isoform 2, although some overlap between both isoforms, hence differentially regulates excitatory synaptic transmission (PubMed : 33262245). In hippocampal neuron cell cultures, isoform 1 controls both miniature excitatory postsynaptic current amplitude and frequency (PubMed : 33262245). Isoform 1 may regulate genes involved in hippocampal-dependent memory, leading to positive regulation of memory, contrary to isoform 2 that may decrease memory (PubMed : 33262245).. Isoform 2. Dioxygenase that plays a key role in active DNA demethylation (PubMed : 27916660, PubMed : 36056023). As isoform 1, binds to promoters, particularly to those with high CG content, however displays reduced global chromatin affinity compared with isoform 1, leading to decreased global DNA demethylation compared with isoform 1 (PubMed : 27916660). Contrary to isoform 1, isoform 2 localizes during S phase to sites of ongoing DNA replication in heterochromatin, causing a significant de novo 5hmC formation, globally, and more so in heterochromatin, including LINE 1 interspersed DNA repeats leading to their activation (PubMed : 36056023). In hippocampal neurons, isoform 2 regulates the expression of a unique subset of genes compared with isoform 1, although some overlap between both isoforms, hence differentially regulating excitatory synaptic transmission (PubMed : 33262245). In hippocampal neuron cell cultures, isoform 2 controls miniature excitatory postsynaptic current frequency, but not amplitude (PubMed : 33262245). Isoform 2 may regulate genes involved in hippocampal-dependent memory, leading to negative regulation of memory, contrary to isoform 1 that may improve memory (PubMed : 33262245). In immature and partially differentiated gonadotrope cells, represses luteinizing hormone gene LHB expression directly and does not catalyze 5hmC at the gene promoter (PubMed : 28855337).
See full target information Tet1
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Product promise

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