Anti-TET1 antibody [EPR30558-579] - BSA and Azide free

• ChIP-seq

Lab

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

Chromatin was prepared from ES-D3 [D3] cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab325925 [EPR30558-579]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Lab

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

Chromatin was prepared from ES-D3 [D3] cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab325925 [EPR30558-579]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Lab

ChIP-sequencing - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

Chromatin was prepared from ES-D3 [D3] cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab325925 [EPR30558-579]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• WB

Lab

Western blot - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-TET1 antibody [EPR30558-579] (<a href='/en-us/products/primary-antibodies/tet1-antibody-epr30558-579-ab325925'>ab325925</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

F9 transfected with siRNA specifically targeting TET1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa,124 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-TET1 antibody [EPR30558-579] (<a href='/en-us/products/primary-antibodies/tet1-antibody-epr30558-579-ab325925'>ab325925</a>) at 1/1000 dilution

All lanes:

ES-D3 [D3]  (mouse blastocyst-derived embryonic stem cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : A20.

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-TET1 antibody [EPR30558-579] (<a href='/en-us/products/primary-antibodies/tet1-antibody-epr30558-579-ab325925'>ab325925</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa,124 kDa

false

Exposure time: 180s

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab325925 [EPR30558-579]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab325925 [EPR30558-579]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab325925 [EPR30558-579]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• I-ELISA

Lab

Indirect ELISA - Anti-TET1 antibody [EPR30558-579] - BSA and Azide free (AB325941)

This data was developed using ab325925, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab325925 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Mouse TET1; Mouse TET2; Mouse TET3.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with Mouse TET2 and Mouse TET3 by ELISA.