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AB213369

Anti-Tet2 antibody [EPR20546-135]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal TET2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

Kiaa1546, Tet2, Methylcytosine dioxygenase TET2, Protein Ayu17-449

3 Images
Western blot - Anti-Tet2 antibody [EPR20546-135] (AB213369)
  • WB

AbReview78103****

Western blot - Anti-Tet2 antibody [EPR20546-135] (AB213369)

Blocking buffer : 5% milk.

All lanes:

Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/2000 dilution

Lane 1:

Wild-Type Mouse embryonic stem cells (mESCs) at 20 µg

Lane 2:

Tet1/2/3 Triple-Knockout (Tet-TKOs) Mouse embryonic stem cells (mESCs) at 20 µg

Secondary

All lanes:

Rat anti-mouse HRP Conjugate at 1/4000 dilution

Predicted band size: 223 kDa

Observed band size: 250 kDa,65 kDa

false

Exposure time: 10s

Image courtesy of Dr Hugo Sepulveda in Prof Anjana Rao’s laboratory (La Jolla Institute for Immunology)

Western blot - Anti-Tet2 antibody [EPR20546-135] (AB213369)
  • WB

Lab

Western blot - Anti-Tet2 antibody [EPR20546-135] (AB213369)

Blocking and Diluting buffer : 5% NFDM/TBST

Exposure time : 3 minutes

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution

All lanes:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 223 kDa

Observed band size: 257 kDa

false

Western blot - Anti-Tet2 antibody [EPR20546-135] (AB213369)
  • WB

Collaborator

Western blot - Anti-Tet2 antibody [EPR20546-135] (AB213369)

Blocking and Diluting buffer : 5% NFDM/TBST

This western was done using lysates from a Tet2/3 DKO B-cell model developed by Dr Vipul Shukla and Dr Chan-Wang Lio, in Prof Anjana Rao's lab.

All lanes:

Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution

Lane 1:

Wild-type B-cell lysate

Lane 2:

Tet2/3 DKO B-cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 223 kDa

Observed band size: 257 kDa

false

Exposure time: 60s

Image courtesy of Dr Hugo Sepulveda in Prof Anjana Rao’s laboratory (La Jolla Institute for Immunology)

  • Carrier free

    Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20546-135

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tet2 also known as ten-eleven translocation methylcytosine dioxygenase 2 is an enzyme involved in DNA demethylation. It has a molecular weight of approximately 224 kDa. This enzyme catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine an important step in DNA demethylation processes. Tet2 expression occurs in various tissues with higher levels in hematopoietic cells. Its role in modification of DNA has made it a significant subject of study in epigenetic regulation.
Biological function summary

Tet2 participates in the regulation of gene expression through its impact on DNA methylation status. By promoting DNA demethylation Tet2 influences transcriptional networks that are vital for cell differentiation and development. The enzyme functions as part of a larger complex with cofactors including ascorbic acid which it requires for full enzymatic activity. Its actions affect the regulation of important processes such as self-renewal and differentiation in hematopoietic stem cells.

Pathways

The enzymatic activity of Tet2 plays a role in epigenetic pathways involved in hematopoiesis and immune function. In particular Tet2 interacts with pathways regulating chromatin structure and transcriptional control. It associates with proteins like TET1 and TET3 as they share roles in the regulation of 5-hydroxymethylcytosine levels. Through these mechanisms Tet2 contributes significantly to the maintenance of normal hematopoietic and immune cell function.

Alterations in Tet2 activity have been linked to hematological malignancies such as myelodysplastic syndromes and acute myeloid leukemia. Loss of function mutations in Tet2 lead to disruptions in hematopoietic differentiation contributing to disease pathology. Tet2 mutations can affect interactions with proteins such as DNMT3A another promoter of DNA methylation which further influence the disease course. Understanding Tet2's role in these disorders may offer potential therapeutic insights.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.
See full target information Tet2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical and translational hepatology 12:917-929 PubMed39544244

2024

GPX4 Promoter Hypermethylation Induced by Ischemia/Reperfusion Injury Regulates Hepatocytic Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Bai,Peilun Xiao,Yuting Chen,Fangfang Chu,Yue Jiao,Jiaqi Fan,Yuexia Zhang,Jiao Liu,Jiying Jiang,Shuna Yu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306294 PubMed38757379

2024

Prenatal 1-Nitropyrene Exposure Causes Autism-Like Behavior Partially by Altering DNA Hydroxymethylation in Developing Brain.

Applications

WB

Species

Mouse

Ting Zhao,Cheng-Qing Huang,Yi-Hao Zhang,Yan-Yan Zhu,Xiao-Xi Chen,Tao Wang,Jing Shao,Xiu-Hong Meng,Yichao Huang,Hua Wang,Hui-Li Wang,Bo Wang,De-Xiang Xu

Nature aging 3:64-81 PubMed36743663

2023

The age of bone marrow dictates the clonality of smooth muscle-derived cells in atherosclerotic plaques.

Applications

WB

Species

Mouse

Inamul Kabir,Xinbo Zhang,Jui M Dave,Raja Chakraborty,Rihao Qu,Rachana R Chandran,Aglaia Ntokou,Eunate Gallardo-Vara,Binod Aryal,Noemi Rotllan,Rolando Garcia-Milian,John Hwa,Yuval Kluger,Kathleen A Martin,Carlos Fernández-Hernando,Daniel M Greif
View all publications
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