Anti-TET2 antibody [EPR26694-93] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TET2 antibody [EPR26694-93] - BSA and Azide free (AB309482)

This data was developed using ab309481, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling TET2 with ab309481 at 1/50 (10.3 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear staining in F9 cell line, and no staining in NIH/3T3 cell line.Negative control : NIH/3T3.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TET2 antibody [EPR26694-93] - BSA and Azide free (AB309482)

This data was developed using ab309481, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast, Left) / F9 (mouse embryonal carcinoma epithelial cell, Right) cells labelling TET2 with ab309481 at 1/5000 dilution (0.01 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : NIH/3T3 (CST Datasheet).

• IP

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Immunoprecipitation - Anti-TET2 antibody [EPR26694-93] - BSA and Azide free (AB309482)

This data was developed using ab309481, the same antibody clone in a different buffer formulation. TET2 was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate with ab309481 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate Lane 2 : ab309481 IP in F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309481 in F9 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-TET2 antibody [EPR26694-93] (<a href='/en-us/products/primary-antibodies/tet2-antibody-epr26694-93-ab309481'>ab309481</a>) at 1/30 dilution

All lanes:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 260 kDa

false

Exposure time: 180s

• WB

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Western blot - Anti-TET2 antibody [EPR26694-93] - BSA and Azide free (AB309482)

This data was developed using ab309481, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : NIH/3T3. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34667079). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-TET2 antibody [EPR26694-93] (<a href='/en-us/products/primary-antibodies/tet2-antibody-epr26694-93-ab309481'>ab309481</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-TET2 antibody [EPR26694-93] - BSA and Azide free (AB309482)

This data was developed using ab309481, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34667079). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-TET2 antibody [EPR26694-93] (<a href='/en-us/products/primary-antibodies/tet2-antibody-epr26694-93-ab309481'>ab309481</a>) at 1/1000 dilution

All lanes:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa

false

Exposure time: 180s