Anti-Tet2 antibody - N-terminal

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Tet2 antibody - N-terminal (AB230358)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling TET2 protein at nucleus using ab230358 at a 1/500 dilution (Green). Alpha Tubulin, Cytoskeleton Marker was counterstained in red.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tet2 antibody - N-terminal (AB230358)

Paraffin-embedded human cervical carcinoma tissue stained for Tet2 using ab230358 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tet2 antibody - N-terminal (AB230358)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for TET2 protein at nucleus with ab230358 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ChIP

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ChIP - Anti-Tet2 antibody - N-terminal (AB230358)

Cross-linked ChIP was performed with U-2 OS (human bone osteosarcoma epithelial cell line) chromatin extract and 5 μg of either control rabbit IgG or ab230358. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

• IP

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Immunoprecipitation - Anti-Tet2 antibody - N-terminal (AB230358)

Tet2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 5 μg ab230358. Western blot was performed from the immunoprecipitate using ab230358. Anti rabbit IgG antibody was used as secondary antibody.

Lane 1 : Control IgG IP in HEK-293T whole cell lysate.

Lane 2 : ab230358 IP in HEK-293T whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Tet2 antibody - N-terminal (ab230358)

Predicted band size: 223 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tet2 antibody - N-terminal (AB230358)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue stained for TET2 protein at nucleus with ab230358 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

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Western blot - Anti-Tet2 antibody - N-terminal (AB230358)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Tet2 antibody - N-terminal (ab230358) at 1/500 dilution

Lane 1:

Wild-type TET2 HeLa cell extracts at 30 µg

Lane 2:

Knockout TET2 HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 160 kDa

false

• WB

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Western blot - Anti-Tet2 antibody - N-terminal (AB230358)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Tet2 antibody - N-terminal (ab230358) at 1/500 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 160 kDa

false

• WB

Supplier Data

Western blot - Anti-Tet2 antibody - N-terminal (AB230358)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Tet2 antibody - N-terminal (ab230358) at 1/5000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

DDDDK-human Tet2-transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 223 kDa

false