Rabbit Polyclonal TET3 antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TET3 aa 1450-1700.
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Publications
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- Frontiers in cardiovascular medicine 9:105369720225mC modification patterns provide novel direction for early acute myocardial infarction detection and personalized therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiqun Guo,Hua Jiang,Jinlong Wang,Ping Li,Xiaoquan Zeng,Tao Zhang,Jianyi Feng,Ruqiong Nie,Yulong Liu,Xiaobian Dong,Qingsong HuPubMed 36620624
- Open medicine (Warsaw, Poland) 17:1883-18952022TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaoling Zhu,Bowen Shi,Chunguang Li,Shuchang XuPubMed 36518116
- Cell & bioscience 12:252022Regulation of paternal 5mC oxidation and H3K9me2 asymmetry by ERK1/2 in mouse zygotes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaobao Chen,Mingtian Deng,Meng-Hao Pan,Shao-Chen Sun,Honglin LiuPubMed 35255956
- Molecular psychiatry :2020Tet3 ablation in adult brain neurons increases anxiety-like behavior and regulates cognitive function in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCláudia Antunes,Jorge D Da Silva,Sónia Guerra-Gomes,Nuno D Alves,Fábio Ferreira,Eduardo Loureiro-Campos,Miguel R Branco,Nuno Sousa,Wolf Reik,Luísa Pinto,C Joana MarquesPubMed 32103150
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TET3 aa 1450-1700. The exact immunogen used to generate this antibody is proprietary information. Database link O43151
Reactivity data
Select an application| IP | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034).
Alternative names
KIAA0401, TET3, Methylcytosine dioxygenase TET3
Recommended products
Rabbit Polyclonal TET3 antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TET3 aa 1450-1700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TET3 aa 1450-1700. The exact immunogen used to generate this antibody is proprietary information. Database link O43151
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET3 antibody (ab153724)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining TET3 protein at nucleus with ab153724 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, 15min.
Immunocytochemistry/ Immunofluorescence - Anti-TET3 antibody (ab153724)
Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling TET3 protein at cytoplasm and nucleus (green) with ab153724 at 1/400 dilution. Red: phalloidin, a cytoskeleton marker, at 1/50 dilution.
Immunoprecipitation - Anti-TET3 antibody (ab153724)
Immunoprecipitation of TET3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells.
IP Sample:
Lane 1: Transfected HEK-293T cell lysate.
Lane 2: Control with 2 μg of preimmune rabbit IgG
Lane 3: Immunoprecipitate of TET3-transfected HEK-293T cells using ab153724 at 2 μg.5% SDS-PAGE
Detection using ab153724 at 1/1000 dilution, followed by anti-rabbit IgG antibody.All lanes: Immunoprecipitation - Anti-TET3 antibody (ab153724)
Predicted band size: 179 kDa
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