AB153724

Anti-TET3 antibody

Name: Anti-TET3 antibody (ab153724) | Abcam
Brand: Abcam Limited
SKU: AB153724
Price: 490 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal TET3 antibody. Suitable for WB, IP, ICC/IF and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TET3 aa 1450 to C-terminus.

View Alternative Names

CXXC10, KIAA0401, TET3, Methylcytosine dioxygenase TET3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TET3 antibody (AB153724)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TET3 antibody (AB153724)

Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling TET3 protein at cytoplasm and nucleus (green) with ab153724 at 1/400 dilution. Red : phalloidin, a cytoskeleton marker, at 1/50 dilution.

Unknown

Immunoprecipitation - Anti-TET3 antibody (AB153724)

Immunoprecipitation of TET3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells.

IP Sample :
Lane 1 : Transfected HEK-293T cell lysate.
Lane 2 : Control with 2 μg of preimmune rabbit IgG
Lane 3 : Immunoprecipitate of TET3-transfected HEK-293T cells using ab153724 at 2 μg.

5% SDS-PAGE

Detection using ab153724 at 1/1000 dilution, followed by anti-rabbit IgG antibody.

All lanes:

Immunoprecipitation - Anti-TET3 antibody (ab153724)

Predicted band size: 179 kDa

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Supplier Data

Western blot - Anti-TET3 antibody (AB153724)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-TET3 antibody (ab153724) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

TET1 transfected 293T whole cell extracts at 30 µg

Lane 3:

TET2 transfected 293T whole cell extracts at 30 µg

Lane 4:

TET3 transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 220 kDa

true

Supplier Data

Western blot - Anti-TET3 antibody (AB153724)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-TET3 antibody (ab153724) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 220 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human TET3 aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/500 - 1/1000", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed : 31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed : 23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed : 23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed : 29276034).

See full target information TET3

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:1362 PubMed39779759

2025

Tryptophan metabolism-related gene CYP1B1 serves as a shared biomarker for both Parkinson's disease and insomnia.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Li,Wen-Kai Yu,Jing-Hao Wu,Wen-Jun He,Yu-Nan Cheng,Kai Gao,Yi-Han Wei,Yu-Sheng Li

Frontiers in pharmacology 15:1418456 PubMed39104395

2024

Pan-cancer analyses reveal the molecular and clinical characteristics of TET family members and suggests that TET3 maybe a potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Zhang,Jie Zheng,Jin Liu,Yanxia Li,Guoqiang Xing,Shupeng Zhang,Hekai Chen,Jian Wang,Zhijiang Shao,Yongyuan Li,Zhongmin Jiang,Yingzi Pan,Xiaozhi Liu,Ping Xu,Wenhan Wu

Advanced biology 9:e2400145 PubMed39007414

2024

TET3 Contributes to Exercise-Induced Functional Axon Regeneration and Visual Restoration.

Applications

Unspecified application

Species

Unspecified reactive species

Si Fang,Yunxiang Ji,Yilan Shen,Simin Yang,Hongli Zhang,Wenfeng Xin,Weidong Shi,Wei Chen

Experimental and therapeutic medicine 25:29 PubMed36561617

2022

Altered genome‑wide hydroxymethylation analysis for neoadjuvant chemoradiotherapy followed by surgery in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjing Zhang,Mingzhu Lu,Jing Zhu,Changsong Zhang,Meihua Wang

Frontiers in cardiovascular medicine 9:1053697 PubMed36620624

2022

5mC modification patterns provide novel direction for early acute myocardial infarction detection and personalized therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Guo,Hua Jiang,Jinlong Wang,Ping Li,Xiaoquan Zeng,Tao Zhang,Jianyi Feng,Ruqiong Nie,Yulong Liu,Xiaobian Dong,Qingsong Hu

Open medicine (Warsaw, Poland) 17:1883-1895 PubMed36518116

2022

TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maoling Zhu,Bowen Shi,Chunguang Li,Shuchang Xu

Cell & bioscience 12:25 PubMed35255956

2022

Regulation of paternal 5mC oxidation and H3K9me2 asymmetry by ERK1/2 in mouse zygotes.

Applications

Unspecified application

Species

Unspecified reactive species

Baobao Chen,Mingtian Deng,Meng-Hao Pan,Shao-Chen Sun,Honglin Liu

Molecular psychiatry : PubMed32103150

2020

Tet3 ablation in adult brain neurons increases anxiety-like behavior and regulates cognitive function in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cláudia Antunes,Jorge D Da Silva,Sónia Guerra-Gomes,Nuno D Alves,Fábio Ferreira,Eduardo Loureiro-Campos,Miguel R Branco,Nuno Sousa,Wolf Reik,Luísa Pinto,C Joana Marques

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TET3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TET3 antibody (AB153724)

Immunoprecipitation - Anti-TET3 antibody (AB153724)

Western blot - Anti-TET3 antibody (AB153724)

Western blot - Anti-TET3 antibody (AB153724)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise