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AB278064

Anti-Tetanus Toxin antibody [TH-11]

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Mouse Monoclonal TETX antibody. Suitable for WB and reacts with Recombinant full length protein - Clostridium tetani samples.

View Alternative Names

CTC_p60, tetX, Tetanus toxin, Tentoxylysin

1 Images
Western blot - Anti-Tetanus Toxin antibody [TH-11] (AB278064)
  • WB

Supplier Data

Western blot - Anti-Tetanus Toxin antibody [TH-11] (AB278064)

Tetanus toxin Heavy (H) and Light (L) chains

All lanes:

Western blot - Anti-Tetanus Toxin antibody [TH-11] (ab278064) at 1/1000 dilution

Lane 1:

Reduced tetanus toxin

Lane 2:

Un-reduced tetanus toxin

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TH-11

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Applications

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Clostridium tetani": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 5 - 8 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tetanus toxin also known as tetanospasmin is a neurotoxic protein produced by the bacterium Clostridium tetani. It has a molecular mass of approximately 150 kDa. Tetanus toxin is expressed during bacterial proliferation and is later released during bacterial lysis. The toxin is composed of a heavy and light chain linked by disulfide bonds. The heavy chain aids in the binding of the toxin to nerve terminals while the light chain acts as a protease cleaving synaptobrevin on synaptic vesicle membranes.
Biological function summary

Tetanus toxin disrupts synaptic transmission at the neuromuscular junction by blocking the release of neurotransmitters. This action prevents the normal communication between nerves and muscles leading to characteristic muscle stiffness and spasms. Tetanus toxin is not part of a larger complex but operates at one of the final steps in neurotransmitter release. The light chain's enzymatic activity targets SNARE proteins which are essential for the fusion of synaptic vesicles with neuronal membranes.

Pathways

Tetanus toxin affects the synaptic vesicle cycle by inhibiting vesicle release. Its toxic effects block the exocytosis pathway required for neurotransmitter secretion. Tetanus toxin interferes with pathways involving proteins like SNAP-25 and syntaxin integral to membrane fusion and neurotransmitter release. These disruptions cascade into broader neurological pathways disturbing normal neuronal signaling and leading to the symptoms associated with tetanus.

Tetanus toxin is closely related to tetanus a serious condition characterized by muscular rigidity and spastic paralysis. The disease occurs when the toxin spreads from a wound site through the body. Tetanus toxin's activity affects proteins such as synaptobrevin which are critical in neurotransmitter release and therefore contribute to the pathological symptoms of tetanus. The toxin's role in the disease underlines its significance as a target for prevention through immunization with tetanus toxoids.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
See full target information tetX

Additional targets

Tetanus Toxin
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Product promise

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