JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB300089

Anti-Tetanus toxoid antibody [18.2.12.6]

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Recombinant Monoclonal TETX antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Clostridium tetani, Clostridium tetani samples.

View Alternative Names

CTC_p60, tetX, Tetanus toxin, Tentoxylysin

1 Images
Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)
  • WB

Supplier Data

Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)

This clone recognizes the epitope of tetanus toxoid heavy chain fragment C.Blocking and diluting buffer and concentration : 5% NFDM/TBST The 100 kDa band is expected to be the cleaved heavy chain The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10924793). Exposure time : 3 minutes

All lanes:

Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] (ab300089) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag®, whole cell lysate 10 µg

Lane 2:

HEK-293T transfected with Tetanus toxoid expression vector containi a myc-His-tag®, whole cell lysate 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 100 kDa,150 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

18.2.12.6

Isotype

IgG2b

Carrier free

No

Reacts with

Clostridium tetani

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB219834

Anti-Diphtheria Toxin antibody [IGX3492R-3]

20ul selling size|Recombinant

Western blot - Anti-Diphtheria Toxin antibody [IGX3492R-3] (AB219834)

  • 0
  • 0
Primary Antibodies

AB4223

FITC Anti-C1q antibody

Immunocytochemistry - FITC Anti-C1q antibody (AB4223)

  • 4
  • 2
Primary Antibodies

AB214337

Anti-Flavivirus NS1 antibody [D/2/D6/B7]

Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus NS1 antibody [D/2/D6/B7] (AB214337)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Clostridium tetani": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 31.25 ng/ml</p>" }, "Transfected cell lysate - Clostridium tetani": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tetanus Toxoid often referred to as TT is an inactivated toxin used in vaccines to prevent tetanus. It originates from the bacterium Clostridium tetani which produces the neurotoxin tetanospasmin. This toxoid has a mass of approximately 150 kDa and is conventionally administered as a vaccine in humans and animals. It triggers an immune response without causing disease as it's a detoxified version of the toxin. Tetanus toxoid is expressed in a laboratory setting where it undergoes chemical treatment to eliminate toxicity while maintaining its antigenicity.
Biological function summary

Tetanus toxoid stimulates the immune system to produce antibodies against the active tetanus toxin tetanospasmin. It doesn't form part of any specific protein complexes but functions independently to evoke humoral immunity. The binding of these antibodies to tetanospasmin neutralizes its effect thereby preventing the inhibition of neurotransmitter release. This process is important for avoiding the spasms and muscle rigidity associated with tetanus. The immune response it provokes includes the activation of B cells which differentiate into plasma cells that produce anti-tetanus antibodies.

Pathways

Tetanus toxoid plays an integral part in the adaptive immune response. It functions within the humoral immune pathway by stimulating the Th2 response. This activation is associated with B-cell proliferation and differentiation into antibody-secreting cells which provide long-term immunity. The vaccine-related immune response connects to key immunological proteins like CD4+ T-helper cells which play a central role in modulating the immune system's response to vaccines.

The tetanus toxoid vaccine is directly related to preventing tetanus an acute often fatal infection marked by severe muscle contractions. By inducing immunity tetanus toxoid plays a preventive role against tetanospasmin-mediated dysfunction in the nervous system. Additionally the concept of herd immunity associated with tetanus prevention helps in protecting community members including those unvaccinated. No other direct protein associations in disorders are typically highlighted but successful immunization inherently aids in broader public health by reducing incidences of infection.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
See full target information tetX
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com