AB302643

Anti-TetR antibody [31B3] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal TETR2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Escherichia coli, Transfected cell line - Escherichia coli samples.

View Alternative Names

Tetracycline repressor protein class B from transposon Tn10, tetR

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TetR antibody [31B3] - BSA and Azide free (AB302643)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TetR antibody [31B3] - BSA and Azide free (AB302643)

This data was developed using ab302642, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling TetR with ab302642 at 1/50 (17.58 ug/mL) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a TETR2 expression vector containing a myc tag, and no staining in HEK-293T cells transfected with a TETR1 expression vector containing a myc tag. is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

Supplier Data

Western blot - Anti-TetR antibody [31B3] - BSA and Azide free (AB302643)

This data was developed using ab302642, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody has been tested with TetR1(P03038) and TetR2 (P04483) overexpression lysates. It recognized the TetR (P04483). Exposure time : 1 second

All lanes:

Western blot - Anti-TetR antibody [31B3] (<a href='/en-us/products/primary-antibodies/tetr-antibody-31b3-ab302642'>ab302642</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag®, whole cell lysate 20

Lane 2:

HEK-293T transfected with TetR1(P03038) expression vector containi a myc-His-tag®, whole cell lysate 20

Lane 3:

HEK-293T transfected with TetR2 (P04483) expression vector containi a myc-His-tag®, whole cell lysate 4

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 1s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

31B3

Isotype

IgG2b

Carrier free

Yes

Reacts with

Escherichia coli

Applications

WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line - Escherichia coli": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate - Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TetR or tetracycline repressor protein is a regulatory protein that controls the expression of genes involved in resistance to tetracycline. It weighs about 23 kDa. TetR functions as a homodimer binding to substrate DNA sequences to suppress transcription. This repression occurs by binding to the operator sequences of the tetracycline-inducible promoter blocking the progression of RNA polymerase along the DNA strand. TetR mainly operates within the bacterial species Escherichia coli serving as a well-characterized model for gene regulation in prokaryotes.

Biological function summary

TetR plays a critical role in antibiotic resistance mechanisms. When tetracycline is not present TetR maintains its binding to the operator effectively silencing the expression of downstream genes responsible for drug efflux. Upon binding tetracycline the protein undergoes a conformational change that releases the operator sequence allowing the transcription of resistance genes. TetR forms a complex through dimerization which is essential for its function as it binds the operator in pairs.

Pathways

TetR is integral to the regulation of the tetracycline resistance pathway. It is part of a classic feedback loop that includes TetA a protein involved in tetracycline efflux out of the cell. The TetR-TetA regulatory system finely tunes bacterial response to the presence or absence of tetracycline ensuring survival in antibiotic environments. Its interaction with the TetA protein highlights its essential role in regulating efflux pump expression during antibiotic exposure.

TetR connects primarily to antibiotic resistance a major issue in bacterial infections. The misuse of tetracycline in clinical settings emphasizes the significance of TetR in developing tetracycline-resistant bacterial strains. This protein's connection to the TetA efflux pump directly affects the efficacy of tetracycline as an antibacterial treatment. Understanding the TetR regulation mechanism could lead to new therapeutic strategies in combating drug-resistant pathogens particularly methicillin-resistant Staphylococcus aureus (MRSA).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.

See full target information tetR

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TetR antibody [31B3] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-TetR antibody [31B3] - BSA and Azide free (AB302643)

Western blot - Anti-TetR antibody [31B3] - BSA and Azide free (AB302643)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Product promise