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AB122455

Anti-TEX12 antibody

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(2 Publications)

Rabbit Polyclonal TEX12 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Transfected cell lysate - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TEX12 aa 1-100.

View Alternative Names

Testis-expressed protein 12, TEX12

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEX12 antibody (AB122455)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEX12 antibody (AB122455)

ab122455, at 1/200 dilution, staining TEX12 in paraffin embedded Human testis tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-TEX12 antibody (AB122455)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TEX12 antibody (AB122455)

Immunofluorescent staining of Human cell line U-251 MG shows positivity in centrosome. Recommended concentration of ab122455 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-TEX12 antibody (AB122455)
  • WB

Supplier Data

Western blot - Anti-TEX12 antibody (AB122455)

All lanes:

Western blot - Anti-TEX12 antibody (ab122455)

Lane 1:

Marker

Lane 2:

HEK293T cell lysate - vector only transfected

Lane 3:

HEK293T cell lysate - overexpressing TEX12 with a C-terminal myc-DDK tag (~3.1kDa)

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TEX12 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BXU0

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Component of the transverse central element of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (By similarity). Requires SYCP1 in order to be incorporated into the central element (By similarity).
See full target information TEX12

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10096 PubMed39572588

2024

GPATCH11 variants cause mis-splicing and early-onset retinal dystrophy with neurological impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Zanetti,Gwendal Dujardin,Lucas Fares-Taie,Jeanne Amiel,Jérôme E Roger,Isabelle Audo,Matthieu P Robert,Pierre David,Vincent Jung,Nicolas Goudin,Ida Chiara Guerrera,Stéphanie Moriceau,Danielle Amana,Nurit Assia Batzir,Anat Bachar-Zipori,Lina Basel Salmon,Nathalie Boddaert,Sylvain Briault,Ange-Line Bruel,Christine Costet-Fighiera,Luisa Coutinho Santos,Cyril Gitiaux,Karolina Kaminska,Paul Kuentz,Naama Orenstein,Nicole Philip-Sarles,Morgane Plutino,Mathieu Quinodoz,Cristina Santos,Sabine Sigaudy,Mariana Soeiro E Sá,Efrat Sofrin,Ana Berta Sousa,Rui Sousa-Luis,Christel Thauvin-Robinet,Erwin L van Dijk,Khaoula Zaafrane-Khachnaoui,Dinah Zur,Josseline Kaplan,Carlo Rivolta,Jean-Michel Rozet,Isabelle Perrault

Communications biology 4:1371 PubMed34880391

2021

Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12.

Applications

Unspecified application

Species

Unspecified reactive species

Sumit Sandhu,Ieng F Sou,Jill E Hunter,Lucy Salmon,Caroline L Wilson,Neil D Perkins,Neil Hunter,Owen R Davies,Urszula L McClurg
View all publications

Product promise

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