Goat Polyclonal PHEB antibody - conjugated to Texas Red®. Suitable for WB, Dot and reacts with Red Algae samples. Cited in 2 publications.
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- Frontiers in immunology 12:7470322021Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTor Kristian Andersen,Johanna Bodin,Fredrik Oftung,Bjarne Bogen,Siri Mjaaland,Gunnveig GrødelandPubMed 34691056
- NPJ vaccines 4:92019Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTor Kristian Andersen et. al.PubMed 30775000
Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- Texas Red®
- Excitation/Emission
- Ex: 589nm, Em: 615nm
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| WB | Dot | |
|---|---|---|
| Red Algae | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Red Algae | Dilution info - | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Red Algae | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Alternative names
R phycoerythrin alpha subunit, R phycoerythrin beta subunit, rpeA, rpeB
Recommended products
Goat Polyclonal PHEB antibody - conjugated to Texas Red®. Suitable for WB, Dot and reacts with Red Algae samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- Texas Red®
- Excitation/Emission
- Ex: 589nm, Em: 615nm
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification
- Specificity
This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
- Concentration
- Purification notes
Purified by immunoaffinity chromatography using R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
R Phycoerythrin also known as rpeA is a light-harvesting protein found mainly in red algae and cyanobacteria. It plays a mechanical role in collecting light energy and assisting in photosynthesis. R Phycoerythrin features a mass of approximately 240 kDa and expresses primarily within the cells of these aquatic organisms. Its bright red color and fluorescence make it important in various biotechnological applications including bioimaging and flow cytometry.
- Biological function summary
This chromoprotein functions as part of the phycobilisome complex serving as an accessory pigment that captures light and transfers energy to chlorophyll. This complex enhances the efficiency of photosynthesis by expanding the range of light wavelengths that organisms can use. The phycobilisome complex attaches to the thylakoid membranes aiding in the capture and channeling of light energy. Given these capabilities R Phycoerythrin proves to be an important component in maximizing photonic energy conversion within these photosynthetic organisms.
- Pathways
R Phycoerythrin plays an important part in the photosynthetic light-harvesting pathway where it directly transmits energy to photosystem II. It engages with other proteins such as allophycocyanin and chlorophyll-binding proteins to optimize the capture of light. This efficient pathway enables organisms to perform photosynthesis even under low-light conditions ensuring survival in diverse aquatic environments.
- Associated diseases and disorders
Abnormal expressions or malfunctions within this light-harvesting system do not frequently align directly with human diseases. However R Phycoerythrin's analogous pigments in other biological contexts may offer insights into photodamage and oxidative stress-related disorders. While the absence of direct association with specific human disorders limits its direct medical implications its study aids in understanding similar conditions linked to oxidative stress and metabolic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)
Blocking buffer for fluorescent Western blotting used for 30 minutes at room temperature.
Predicted size: 18 kDa for R-Phycoerythrin.
Other bands: R-Phycoerythrin splice varients and isoforms.All lanes: Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734) at 1/1000 dilution
All lanes: R-Phycoerythrin at 0.05 µg
Predicted band size: 18 kDa
Observed band size: 18 kDa
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