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AB34734

Texas Red ® Anti-R Phycoerythrin/rpeA antibody

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(2 Publications)

Goat Polyclonal PHEB antibody - conjugated to Texas Red®. Suitable for WB, Dot and reacts with Red Algae samples. Cited in 2 publications.
1 Images
Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (AB34734)
  • WB

Supplier Data

Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (AB34734)

Blocking buffer for fluorescent Western blotting used for 30 minutes at room temperature.
Predicted size : 18 kDa for R-Phycoerythrin.
Other bands : R-Phycoerythrin splice varients and isoforms.

All lanes:

Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734) at 1/1000 dilution

All lanes:

R-Phycoerythrin at 0.05 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Texas Red®

Excitation/Emission

Ex: 589nm, Em: 615nm

Carrier free

No

Reacts with

Red Algae

Applications

WB, Dot

applications

Specificity

This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Red Algae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
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Product details

Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by immunoaffinity chromatography using R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

R Phycoerythrin also known as rpeA is a light-harvesting protein found mainly in red algae and cyanobacteria. It plays a mechanical role in collecting light energy and assisting in photosynthesis. R Phycoerythrin features a mass of approximately 240 kDa and expresses primarily within the cells of these aquatic organisms. Its bright red color and fluorescence make it important in various biotechnological applications including bioimaging and flow cytometry.
Biological function summary

This chromoprotein functions as part of the phycobilisome complex serving as an accessory pigment that captures light and transfers energy to chlorophyll. This complex enhances the efficiency of photosynthesis by expanding the range of light wavelengths that organisms can use. The phycobilisome complex attaches to the thylakoid membranes aiding in the capture and channeling of light energy. Given these capabilities R Phycoerythrin proves to be an important component in maximizing photonic energy conversion within these photosynthetic organisms.

Pathways

R Phycoerythrin plays an important part in the photosynthetic light-harvesting pathway where it directly transmits energy to photosystem II. It engages with other proteins such as allophycocyanin and chlorophyll-binding proteins to optimize the capture of light. This efficient pathway enables organisms to perform photosynthesis even under low-light conditions ensuring survival in diverse aquatic environments.

Abnormal expressions or malfunctions within this light-harvesting system do not frequently align directly with human diseases. However R Phycoerythrin's analogous pigments in other biological contexts may offer insights into photodamage and oxidative stress-related disorders. While the absence of direct association with specific human disorders limits its direct medical implications its study aids in understanding similar conditions linked to oxidative stress and metabolic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information R Phycoerythrin/rpeA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:747032 PubMed34691056

2021

Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief.

Applications

Unspecified application

Species

Unspecified reactive species

Tor Kristian Andersen,Johanna Bodin,Fredrik Oftung,Bjarne Bogen,Siri Mjaaland,Gunnveig Grødeland

NPJ vaccines 4:9 PubMed30775000

2019

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Tor Kristian Andersen,Peter C Huszthy,Ramakrishna P Gopalakrishnan,Johanne T Jacobsen,Marte Fauskanger,Anders A Tveita,Gunnveig Grødeland,Bjarne Bogen
View all publications
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Product promise

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