AB93808

Anti-TFE3 antibody

Name: Anti-TFE3 antibody (ab93808) | Abcam
Brand: Abcam Limited
SKU: AB93808
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(20 Publications)

Anti-TFE3 antibody (ab93808) is a rabbit polyclonal antibody detecting TFE3 in Western Blot, IP. Suitable for Human.

- Trusted since 2010

View Alternative Names

BHLHE33, TFE3, Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33

2 Images

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Western blot - Anti-TFE3 antibody (AB93808)

All lanes:

Western blot - Anti-TFE3 antibody (ab93808) at 0.04 µg/mL

Lane 1:

HeLa whole lysate at 50 µg

Lane 2:

HeLa whole lysate at 15 µg

Lane 3:

HeLa whole lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Predicted band size: 62 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-TFE3 antibody (AB93808)

1 mg HeLa cell lysate was immunoprecipitated with 3μg/mg ab93808 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitates were analysed by Western blot. Bands were analysed using ab93808 at 0.4μg/ml and developed using cheiluminescence.

All lanes:

Immunoprecipitation - Anti-TFE3 antibody (ab93808)

Predicted band size: 62 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TFE3 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

P19532

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TFE3 antibody (ab93808) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human samples.

What is the molecular weight of TFE3?
Anti-TFE3 (ab93808) specifically detects a band for TFE3 (UniProt: P19532) at a molecular weight of 62kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response (PubMed : 2338243, PubMed : 24448649, PubMed : 29146937, PubMed : 30733432, PubMed : 31672913, PubMed : 37079666). Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (PubMed : 24448649). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation (PubMed : 24448649, PubMed : 31672913, PubMed : 36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity (PubMed : 24448649, PubMed : 31672913, PubMed : 36608670). Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes (PubMed : 24448649). Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB; mTOR-dependent TFE3 cytosolic retention and inactivation promotes exit from pluripotency (By similarity). Required to maintain the naive pluripotent state of hematopoietic stem cell; mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency (PubMed : 30733432). TFE3 activity is also involved in the inhibition of neuronal progenitor differentiation (By similarity). Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue (By similarity). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer (PubMed : 2338243). It also binds very well to a USF/MLTF site (PubMed : 2338243). Promotes TGF-beta-induced transcription of COL1A2; via its interaction with TSC22D1 at E-boxes in the gene proximal promoter (By similarity). May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed : 29146937).

See full target information TFE3

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Translational neurodegeneration 14:31 PubMed40537859

2025

Pathological α-synuclein elicits granulovacuolar degeneration independent of tau.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan J Dues,Madalynn L Erb,Alysa Kasen,Naman Vatsa,Erin T Williams,An Phu Tran Nguyen,Michael X Henderson,Darren J Moore

iScience 28:112122 PubMed40463960

2025

An engineered tumor organoid model reveals cellular identity and signaling trajectories underlying SFPQ-TFE3 driven translocation RCC.

Applications

Unspecified application

Species

Unspecified reactive species

Maroussia M P Ganpat,Francisco Morales-Rodriguez,Nhung Pham,Philip Lijnzaad,Terezinha de Souza,Sepide Derakhshan,Arianna Fumagalli,Peter Zeller,Aleksandra Balwierz,Dilara Ayyildiz,Marry M van den Heuvel-Eibrink,Ronald R de Krijger,Alexander van Oudenaarden,Thanasis Margaritis,Susana M Chuva de Sousa Lopes,Jarno Drost

Cell reports 44:115539 PubMed40222010

2025

TFE3 fusion oncoprotein condensates drive transcriptional reprogramming and cancer progression in translocation renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Choon Leng So,Ye Jin Lee,Bujamin H Vokshi,Wanlu Chen,Binglin Huang,Emily De Sousa,Yangzhenyu Gao,Marie Elena Portuallo,Sumaiya Begum,Kasturee Jagirdar,W Marston Linehan,Vito W Rebecca,Hongkai Ji,Eneda Toska,Danfeng Cai

EMBO molecular medicine 17:1041-1070 PubMed40148585

2025

TFE3 fusions drive oxidative metabolism and ferroptosis resistance in translocation renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Helleux,Guillaume Davidson,Antonin Lallement,Fatima Al Hourani,Alexandre Haller,Isabelle Michel,Anas Fadloun,Christelle Thibault-Carpentier,Xiaoping Su,Véronique Lindner,Thibault Tricard,Hervé Lang,Nizar M Tannir,Irwin Davidson,Gabriel G Malouf

Cell communication and signaling : CCS 23:122 PubMed40050998

2025

TFE3 fusion proteins promote the progression of TFE3 rearranged renal cell carcinoma via enhancing chaperone-mediated lipophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenliang Ma,Yi Chen,Guijuan Chen,Lei Yang,Yanwen Lu,Xiang Dong,Dongmei Li,Weidong Gan

Genes & diseases 12:101429 PubMed39759118

2025

TFE3-mediated neuroprotection: Clearance of aggregated α-synuclein and accumulated mitochondria in the AAV-α-synuclein model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xin He,Mulan Chen,Yepeng Fan,Bin Wu,Zhifang Dong

Genes & development 38:718-737 PubMed39168638

2024

TFEB controls expression of human syncytins during cell-cell fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Meagan N Esbin,Liza Dahal,Vinson B Fan,Joey McKenna,Eric Yin,Xavier Darzacq,Robert Tjian

Experimental & molecular medicine 56:1807-1815 PubMed39085357

2024

Comprehensive molecular characterization of TFE3-rearranged renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Cho-Rong Lee,Jungyo Suh,Dongjun Jang,Bo-Yeong Jin,Jaeso Cho,Moses Lee,Hyungtai Sim,Minyong Kang,Jueun Lee,Ju Hyun Park,Kyoung-Hwa Lee,Geum-Sook Hwang,Kyung Chul Moon,Cheryn Song,Ja Hyeon Ku,Cheol Kwak,Hyeon Hoe Kim,Sung-Yup Cho,Murim Choi,Chang Wook Jeong

NPJ Parkinson's disease 10:133 PubMed39030200

2024

Adult-onset deletion of ATP13A2 in mice induces progressive nigrostriatal pathway dopaminergic degeneration and lysosomal abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Madalynn L Erb,Kayla Sipple,Nathan Levine,Xi Chen,Darren J Moore

Scientific reports 12:6522 PubMed35444208

2022

Glycoprotein nonmetastatic melanoma protein B regulates lysosomal integrity and lifespan of senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masayoshi Suda,Ippei Shimizu,Goro Katsuumi,Chieh Lun Hsiao,Yohko Yoshida,Naomi Matsumoto,Yutaka Yoshida,Akihiro Katayama,Jun Wada,Masahide Seki,Yutaka Suzuki,Shujiro Okuda,Kazuyuki Ozaki,Mayumi Nakanishi-Matsui,Tohru Minamino

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TFE3 antibody

Western blot - Anti-TFE3 antibody (AB93808)

Immunoprecipitation - Anti-TFE3 antibody (AB93808)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (20)

Product promise