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AB314920

Anti-TFEB (phospho S211) antibody [EPR27960-64] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal TFEB phospho S211 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

BHLHE35, TFEB, Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35

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Sandwich ELISA - Anti-TFEB (phospho S211) antibody [EPR27960-64] - BSA and Azide free (Capture) (AB314920)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TFEB (phospho S211) antibody [EPR27960-64] - BSA and Azide free (Capture) (AB314920)

Sandwich ELISA of ab314919 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL. Raji + Torin 1 : Raji treated with 250 nM Torin 1 for 5 hours, whole cell lysate. Serial dilutions of lysates were analyzed.

Sandwich ELISA - Anti-TFEB (phospho S211) antibody [EPR27960-64] - BSA and Azide free (Capture) (AB314920)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TFEB (phospho S211) antibody [EPR27960-64] - BSA and Azide free (Capture) (AB314920)

Raji + Torin 1 : Raji treated with 250 nM Torin 1 for 5 hours, whole cell lysate. Serial dilutions of lysates were analyzed. Raji Control : untreated Raji, whole cell lysate. Serial dilutions of lysates were analyzed.

  • Unconjugated

    Anti-TFEB (phospho S211) antibody [EPR27960-64]

  • Carrier free

    Anti-TFEB (phospho S211) antibody [EPR27960-64] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27960-64

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab314920 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TFEB (phospho S211).
Capture: ab314920, Human TFEB (phospho S211) Capture Antibody (unconjugated)
Detector: ab314921, Human TFEB Detector Antibody (unconjugated)

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response (PubMed : 21617040, PubMed : 22343943, PubMed : 22576015, PubMed : 22692423, PubMed : 25720963, PubMed : 30120233, PubMed : 31672913, PubMed : 32612235, PubMed : 32753672, PubMed : 35662396, PubMed : 36697823, PubMed : 36749723, PubMed : 37079666). Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF (PubMed : 1748288, PubMed : 19556463, PubMed : 29146937). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation (PubMed : 21617040, PubMed : 22343943, PubMed : 22576015, PubMed : 22692423, PubMed : 25720963, PubMed : 32612235, PubMed : 32753672, PubMed : 35662396, PubMed : 36697823). Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity (PubMed : 22343943, PubMed : 22576015, PubMed : 22692423, PubMed : 25720963, PubMed : 32612235, PubMed : 32753672, PubMed : 35662396, PubMed : 36697823). Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes (PubMed : 19556463, PubMed : 22692423). Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed : 29146937). Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy (PubMed : 21617040, PubMed : 22576015, PubMed : 23434374, PubMed : 27278822). In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer (PubMed : 2115126). Plays a role in the signal transduction processes required for normal vascularization of the placenta (By similarity). Involved in the immune response to infection by the bacteria S.aureus, S.typhimurium or S.enterica : infection promotes itaconate production, leading to alkylation, resulting in nuclear localization and transcription factor activity (PubMed : 35662396). Itaconate-mediated alkylation activates TFEB-dependent lysosomal biogenesis, facilitating the bacteria clearance during the antibacterial innate immune response (PubMed : 35662396). In association with ACSS2, promotes the expression of genes involved in lysosome biogenesis and both autophagy upon glucose deprivation (PubMed : 28552616).
See full target information TFEB phospho S211
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