Anti-TFF2 antibody [EPR23231-208]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (AB267474)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TFF2 with ab267474 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on human lung (PMID : 17242463). The section was incubated with ab267474 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (AB267474)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling TFF2 with ab267474 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human stomach (PMID : 17242463) The section was incubated with ab267474 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IP

Unknown

Immunoprecipitation - Anti-TFF2 antibody [EPR23231-208] (AB267474)

TFF2 was immunoprecipitated from 0.35 mg Human stomach lysate with ab267474 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267474 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : Human stomach lysate 10ug

Lane 2 : ab267474 IP in Human stomach lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab267474 in Human stomach lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds

TFF2 is a glycoprotein. The 24 kDa band is likely to be a higher glycosylated TFF2. (PMID : 10716671)

All lanes:

Immunoprecipitation - Anti-TFF2 antibody [EPR23231-208] (ab267474)

Predicted band size: 14 kDa

false

• WB

Lab

Western blot - Anti-TFF2 antibody [EPR23231-208] (AB267474)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

TFF2 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : 10716671)

Negative Control : Human heart (PMID : 10452549)

Exposure time : 37 seconds

All lanes:

Western blot - Anti-TFF2 antibody [EPR23231-208] (ab267474) at 1/1000 dilution

Lane 1:

Human stomach tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

false