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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal TFII I antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | IP | Flow Cyt | WB | |
---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Tested |
Mouse | Predicted | Not recommended | Not recommended | Predicted |
Rat | Predicted | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, SRF-Phox1-interacting protein, Williams-Beuren syndrome chromosomal region 6 protein, BAP-135, BTK-associated protein 135, SPIN, BAP135, WBSCR6, GTF2I
Rabbit Recombinant Monoclonal TFII I antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, SRF-Phox1-interacting protein, Williams-Beuren syndrome chromosomal region 6 protein, BAP-135, BTK-associated protein 135, SPIN, BAP135, WBSCR6, GTF2I
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
EPR7696
Affinity purification Protein A
1.9 x 10-11 M
Blue Ice
-20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-TFII I antibody [EPR7696] (AB134133) at 1/10000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: U937 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Lane 5: 293T cell lysate at 10 µg
All lanes: HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 112 kDa
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TFII I with ab134133 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TFII I with ab134133 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com