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Rabbit Recombinant Monoclonal TFII I antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Predicted
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.

Alternative names

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Rabbit Recombinant Monoclonal TFII I antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR7696

Purification technique

Affinity purification Protein A

Dissociation constant

1.9 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-TFII I antibody [EPR7696] (ab134133), expandable thumbnail

    Western blot - Anti-TFII I antibody [EPR7696] (ab134133)

    All lanes: Western blot - Anti-TFII I antibody [EPR7696] (AB134133) at 1/10000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: U937 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Lane 5: 293T cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 112 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TFII I with ab134133 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TFII I with ab134133 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-TFII I antibody [EPR7696] (ab134133), expandable thumbnail

    OI-RD Scanning - Anti-TFII I antibody [EPR7696] (ab134133)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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