Rabbit Recombinant Monoclonal TFII I antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--western-blot-img161287.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47408.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96098.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640)")
![OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--oi-rd-scanning-img142943.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (AB185640)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
Alternative names
BAP135, WBSCR6, GTF2I, General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, SRF-Phox1-interacting protein, Williams-Beuren syndrome chromosomal region 6 protein, BAP-135, BTK-associated protein 135, SPIN
Recommended products
Rabbit Recombinant Monoclonal TFII I antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7696
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.9 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab185640 is the carrier-free version of Anti-TFII I antibody [EPR7696] ab134133.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--western-blot-img161287.jpg "Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)")
Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
This data was developed using Anti-TFII I antibody [EPR7696] ab134133, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640) at 1/10000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: U937 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Lane 5: 293T cell lysate at 10 µg
SecondaryAll lanes: HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 112 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47408.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
This data was developed using Anti-TFII I antibody [EPR7696] ab134133, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TFII I with Anti-TFII I antibody [EPR7696] ab134133 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96098.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
This data was developed using Anti-TFII I antibody [EPR7696] ab134133, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TFII I with Anti-TFII I antibody [EPR7696] ab134133 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640), expandable thumbnail](https://content.abcam.com/products/images/tfii-i-antibody-epr7696-bsa-and-azide-free-ab185640--oi-rd-scanning-img142943.jpg "OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)")
OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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