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Rabbit Monoclonal TFIIB antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

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Images

Immunoprecipitation - Anti-TFIIB antibody [EP4588] (AB109518), expandable thumbnail
  • Western blot - Anti-TFIIB antibody [EP4588] (AB109518), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] (AB109518), expandable thumbnail
  • Western blot - Anti-TFIIB antibody [EP4588] (AB109518), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (AB109518), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Tested
Not recommended
Tested
Tested
Tested
Rat
Expected
Not recommended
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100.00000 - 1/250.00000
Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100.00000 - 1/250.00000
Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10.00000 - 1/100.00000
Notes

-

Species
Human
Dilution info
1/10.00000 - 1/100.00000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Species
Rat
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Species
Human
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10.00000 - 1/100.00000
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
1/10.00000 - 1/100.00000
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329). Modulates transcription start site selection (PubMed:10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).

Alternative names

TF2B, TFIIB, GTF2B, Transcription initiation factor IIB, General transcription factor TFIIB, S300-II

Recommended products

Rabbit Monoclonal TFIIB antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP4588
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518), expandable thumbnail

    Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518)

    ab109518 (purified) at 1/500 dilution (0.16 ©:g/ml) immunoprecipitating TFIIB in Jurkat whole cell lysate .
    Lane 1 (input): Jurkat(Human T cell leukemia T lymphocyte) whole cell lysate 10©:g
    Lane 2 (+): ab109518 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109518 in Jurkat whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .

    All lanes: Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518)

    Predicted band size: 34 kDa

  • Western blot - Anti-TFIIB antibody [EP4588] (ab109518), expandable thumbnail

    Western blot - Anti-TFIIB antibody [EP4588] (ab109518)

    All lanes: Western blot - Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 2: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 34 kDa

    Observed band size: 35 kDa

  • Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] (ab109518), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] (ab109518)

    Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling TFIIB with ab109518 at 1/20 dilution (4.05 ©:g/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor� 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

  • Western blot - Anti-TFIIB antibody [EP4588] (ab109518), expandable thumbnail

    Western blot - Anti-TFIIB antibody [EP4588] (ab109518)

    All lanes: Western blot - Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

    Lane 1: HepG2 cell lyaste at 10 µg

    Lane 2: HeLa cell lyaste at 10 µg

    Lane 3: Jurkat cell lyaste at 10 µg

    Lane 4: K562 cell lyaste at 10 µg

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (ab109518), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (ab109518)

    ab109518, at a 1/100 dilution, staining TFIIB in paraffin embedded Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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