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AB109518

Anti-TFIIB antibody [EP4588]

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(1 Publication)

Rabbit Monoclonal TFIIB antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

TF2B, TFIIB, GTF2B, Transcription initiation factor IIB, General transcription factor TFIIB, S300-II

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (AB109518)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (AB109518)

ab109518, at a 1/100 dilution, staining TFIIB in paraffin embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] (AB109518)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] (AB109518)

Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling TFIIB with ab109518 at 1/20 dilution (4.05 © : g/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

Immunoprecipitation - Anti-TFIIB antibody [EP4588] (AB109518)
  • IP

Unknown

Immunoprecipitation - Anti-TFIIB antibody [EP4588] (AB109518)

ab109518 (purified) at 1/500 dilution (0.16 © : g/ml) immunoprecipitating TFIIB in Jurkat whole cell lysate .
Lane 1 (input) : Jurkat(Human T cell leukemia T lymphocyte) whole cell lysate 10© : g
Lane 2 (+) : ab109518 & Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109518 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM /TBST .

All lanes:

Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518)

Predicted band size: 34 kDa

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Western blot - Anti-TFIIB antibody [EP4588] (AB109518)
  • WB

Unknown

Western blot - Anti-TFIIB antibody [EP4588] (AB109518)

All lanes:

Western blot - Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

Lane 1:

HepG2 cell lyaste at 10 µg

Lane 2:

HeLa cell lyaste at 10 µg

Lane 3:

Jurkat cell lyaste at 10 µg

Lane 4:

K562 cell lyaste at 10 µg

Predicted band size: 34 kDa

false

Western blot - Anti-TFIIB antibody [EP4588] (AB109518)
  • WB

Lab

Western blot - Anti-TFIIB antibody [EP4588] (AB109518)

All lanes:

Western blot - Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

false

  • Carrier free

    Anti-TFIIB antibody [EP4588] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4588

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB109106

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Western blot - Anti-TFIIB antibody [EPR4589] (AB109106)

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

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Anti-TFIIF antibody

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Primary Antibodies

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Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade

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Primary Antibodies

AB63766

Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (AB63766)

  • 5
  • 7
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed : 12931194, PubMed : 1517211, PubMed : 1876184, PubMed : 1946368, PubMed : 27193682, PubMed : 3029109, PubMed : 3818643, PubMed : 7601352, PubMed : 8413225, PubMed : 8515820, PubMed : 8516311, PubMed : 8516312, PubMed : 9420329). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed : 8413225, PubMed : 8504927, PubMed : 8515820, PubMed : 8516311, PubMed : 8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed : 7601352). Associates with chromatin to core promoter-specific regions (PubMed : 12931194, PubMed : 24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed : 10619841, PubMed : 16230532, PubMed : 7675079, PubMed : 9420329). Modulates transcription start site selection (PubMed : 10318856). Also exhibits autoacetyltransferase activity that contributes to the activated transcription (PubMed : 12931194).
See full target information GTF2B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40704991

2025

Rapid and inducible mislocalization of endogenous TDP43 in a novel human model of amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Ganssauge,Sophie Hawkins,Seema Chandramohan Namboori,Szi Kay Leung,Jonathan Mill,Akshay Bhinge
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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