Anti-TFIIB antibody [EP4588] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] - BSA and Azide free (AB284785)

This data was developed using ab109518, the same antibody clone in a different buffer formulation.ab109518, at a 1/100 dilution, staining TFIIB in paraffin embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] - BSA and Azide free (AB284785)

This data was developed using ab109518, the same antibody clone in a different buffer formulation.Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling TFIIB with ab109518 at 1/20 dilution (4.05 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

• WB

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Western blot - Anti-TFIIB antibody [EP4588] - BSA and Azide free (AB284785)

This data was developed using ab109518, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TFIIB antibody [EP4588] (<a href='/en-us/products/primary-antibodies/tfiib-antibody-ep4588-ab109518'>ab109518</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lyaste at 10 µg

Lane 2:

HeLa cell lyaste at 10 µg

Lane 3:

Jurkat cell lyaste at 10 µg

Lane 4:

K562 cell lyaste at 10 µg

Predicted band size: 34 kDa

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• WB

Lab

Western blot - Anti-TFIIB antibody [EP4588] - BSA and Azide free (AB284785)

This data was developed using ab109518, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TFIIB antibody [EP4588] (<a href='/en-us/products/primary-antibodies/tfiib-antibody-ep4588-ab109518'>ab109518</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

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• IP

Unknown

Immunoprecipitation - Anti-TFIIB antibody [EP4588] - BSA and Azide free (AB284785)

This data was developed using ab109518, the same antibody clone in a different buffer formulation.

ab109518 (purified) at 1/20 dilution (0.16 μg/ml) immunoprecipitating TFIIB in Jurkat whole cell lysate .
Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab109518 & Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109518 in Jurkat whole cell lysate.
For western blotting, ab109518 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-TFIIB antibody [EP4588] (<a href='/en-us/products/primary-antibodies/tfiib-antibody-ep4588-ab109518'>ab109518</a>)

Predicted band size: 34 kDa

false