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AB28179

Anti-TFIIF antibody

5

(2 Reviews)

|

(6 Publications)

Rabbit Polyclonal GTF2F1 antibody. Suitable for ChIP and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GTF2F1.

View Alternative Names

RAP74, GTF2F1, General transcription factor IIF subunit 1, General transcription factor IIF 74 kDa subunit, Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74, TFIIF-alpha

1 Images
ChIP - Anti-TFIIF antibody (AB28179)
  • ChIP

Unknown

ChIP - Anti-TFIIF antibody (AB28179)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25μg of chromatin, 8μg of ab28179 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GTF2F1.

P35269

Specificity

This antibody reacts with the RAP74 subunit of TFIIF.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5-10 µg for 25 µg chromatin", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.9 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
See full target information GTF2F1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Apoptosis : an international journal on programmed cell death 27:762-777 PubMed35779185

2022

Hypoxic condition induced H3K27me3 modification of the LncRNA Tmem235 promoter thus supporting apoptosis of BMSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhang,Hong Luo,Wuxun Peng,Lei Wang,Tao Wang,Zhihong Xie,Jian Zhang,Wentao Dong,Xiaohan Zheng,Gang Liu,Xuesong Zhu,Qinglin Kang,Xiaobin Tian

Molecular cell 81:3110-3127.e14 PubMed34233157

2021

Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination.

Applications

Unspecified application

Species

Unspecified reactive species

Ashwin Narain,Pranjali Bhandare,Bikash Adhikari,Simone Backes,Martin Eilers,Lars Dölken,Andreas Schlosser,Florian Erhard,Apoorva Baluapuri,Elmar Wolf

The EMBO journal 40:e102509 PubMed34155658

2021

The SAGA complex regulates early steps in transcription via its deubiquitylase module subunit USP22.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Stanek,Victoria J Gennaro,Mason A Tracewell,Daniela Di Marcantonio,Kristen L Pauley,Sabrina Butt,Christopher McNair,Feng Wang,Andrew V Kossenkov,Karen E Knudsen,Tauseef Butt,Stephen M Sykes,Steven B McMahon

Molecular cell 72:836-848.e7 PubMed30415952

2018

MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Manpreet Kalkat,Diana Resetca,Corey Lourenco,Pak-Kei Chan,Yong Wei,Yu-Jia Shiah,Natasha Vitkin,Yufeng Tong,Maria Sunnerhagen,Susan J Done,Paul C Boutros,Brian Raught,Linda Z Penn

Cell reports 2:722-31 PubMed23041311

2012

DSIF restricts NF-κB signaling by coordinating elongation with mRNA processing of negative feedback genes.

Applications

ChIP, WB

Species

Human, Human

Gil Diamant,Liat Amir-Zilberstein,Yuki Yamaguchi,Hiroshi Handa,Rivka Dikstein

PloS one 7:e41152 PubMed22815947

2012

Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Hogel,Robert B Laprairie,Eileen M Denovan-Wright
View all publications
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Product promise

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