Rabbit Polyclonal GTF2F1 antibody. Suitable for ChIP and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GTF2F1.
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Publications
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- Molecular cell 81:3110-3127.e142021Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshwin Narain,Pranjali Bhandare,Bikash Adhikari,Simone Backes,Martin Eilers,Lars Dölken,Andreas Schlosser,Florian Erhard,Apoorva Baluapuri,Elmar WolfPubMed 34233157
- The EMBO journal 40:e1025092021The SAGA complex regulates early steps in transcription via its deubiquitylase module subunit USP22.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy J Stanek,Victoria J Gennaro,Mason A Tracewell,Daniela Di Marcantonio,Kristen L Pauley,Sabrina Butt,Christopher McNair,Feng Wang,Andrew V Kossenkov,Karen E Knudsen,Tauseef Butt,Stephen M Sykes,Steven B McMahonPubMed 34155658
- Molecular cell 72:836-848.e72018MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManpreet Kalkat et. al.PubMed 30415952
- Cell reports 2:722-312012DSIF restricts NF-κB signaling by coordinating elongation with mRNA processing of negative feedback genes.
- PloS one 7:e411522012Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Hogel et. al.PubMed 22815947
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.9
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human GTF2F1. Database link P35269
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Reactivity data
Select an application| ChIP | |
|---|---|
| Human | Tested |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-10 µg chromatin for 25 µg chromatin | Notes - |
Target data
Function
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
Alternative names
RAP74, GTF2F1, General transcription factor IIF subunit 1, General transcription factor IIF 74 kDa subunit, Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74, TFIIF-alpha
Recommended products
Rabbit Polyclonal GTF2F1 antibody. Suitable for ChIP and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GTF2F1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.9
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human GTF2F1. Database link P35269
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts with the RAP74 subunit of TFIIF.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
ChIP - Anti-TFIIF antibody (ab28179)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25μg of chromatin, 8μg of ab28179 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
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