JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB264567

Anti-TFPI antibody [EPR22977-16] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TFPI antibody. Carrier free. Suitable for IP, Flow Cyt, WB and reacts with Human samples.

View Alternative Names

LACI, TFPI1, TFPI, Tissue factor pathway inhibitor, Extrinsic pathway inhibitor, Lipoprotein-associated coagulation inhibitor, EPI

2 Images
Flow Cytometry - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (AB264567)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (AB264567)

Flow cytometric analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling TFPI with ab260041 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260041).

Immunoprecipitation - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (AB264567)
  • IP

Unknown

Immunoprecipitation - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (AB264567)

TFPI was immunoprecipitated from 0.35 mg human placenta lysate 10μg with ab260041 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260041 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human placenta lysate 10μg

Lane 2 : ab260041 IP in human placenta lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab260041 in human placenta lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260041).

All lanes:

Immunoprecipitation - Anti-TFPI antibody [EPR22977-16] (<a href='/en-us/products/primary-antibodies/tfpi-antibody-epr22977-16-ab260041'>ab260041</a>)

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22977-16

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB239516

Anti-TFPI antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI antibody [C1] (AB239516)

  • 0
  • 0
Primary Antibodies

AB134151

Anti-TFPI antibody [EPR7941]

RabMAb|Recombinant

Western blot - Anti-TFPI antibody [EPR7941] (AB134151)

  • 0
  • 0
Primary Antibodies

AB264568

Anti-TFPI antibody [EPR22977-133] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TFPI antibody [EPR22977-133] - BSA and Azide free (AB264568)

  • 0
  • 0
Primary Antibodies

AB260042

Anti-TFPI antibody [EPR22977-133]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TFPI antibody [EPR22977-133] (AB260042)

  • 0
  • 0
Primary Antibodies

AB248703

Anti-TFPI antibody [EPR7941] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-TFPI antibody [EPR7941] - BSA and Azide free (AB248703)

  • 0
  • 0

Complementary Products

Assay Kits

AB274392

Human TFPI ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TFPI ELISA Kit (AB274392)

  • 0
  • 0
Primary Antibodies

AB109189

Anti-Thrombomodulin antibody [EPR4051]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

  • 4
  • 4
Primary Antibodies

AB189483

Anti-Tissue Factor antibody [EPR18160-175]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

  • 0
  • 0
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 17
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab264567 is the carrier-free version of ab260041.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
See full target information TFPI
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com