AB134151

Anti-TFPI antibody [EPR7941]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TFPI antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LACI, TFPI1, TFPI, Tissue factor pathway inhibitor, Extrinsic pathway inhibitor, Lipoprotein-associated coagulation inhibitor, EPI

3 Images

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Western blot - Anti-TFPI antibody [EPR7941] (AB134151)

All lanes:

Western blot - Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Lane 4:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 35 kDa

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Lab

Western blot - Anti-TFPI antibody [EPR7941] (AB134151)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 40-45 kDa

false

Lab

Western blot - Anti-TFPI antibody [EPR7941] (AB134151)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 40-45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7941

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

See full target information TFPI

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virulence 9:724-737 PubMed29473457

2018

An ecoimmunological approach to study evolutionary and ancient links between coagulation, complement and Innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Praveen Papareddy,Gopinath Kasetty,Saud Alyafei,Emanuel Smeds,Outi M H Salo-Ahen,Stefan R Hansson,Arne Egesten,Heiko Herwald

View all publications

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