Rabbit Recombinant Monoclonal TFPI2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Images
![Western blot - Anti-TFPI2 antibody [EPR14442] (AB186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--western-blot-img146830.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TFPI2 antibody [EPR14442] (AB186747)")
![Western blot - Anti-TFPI2 antibody [EPR14442] (AB186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--western-blot-img57556.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TFPI2 antibody [EPR14442] (AB186747)")
![Western blot - Anti-TFPI2 antibody [EPR14442] (AB186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--western-blot-img104340.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TFPI2 antibody [EPR14442] (AB186747)")
![Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (AB186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--immunoprecipitation-img44227.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (AB186747)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] (AB186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113689.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] (AB186747)")
Publications
Filter by
- Journal of inflammation (London, England) 20:352023The TFPI2-PPARγ axis induces M2 polarization and inhibits fibroblast activation to promote recovery from post-myocardial infarction in diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengqi Guo,Zongyi Xia,Yefeng Hong,Hongwei Ji,Fuhai Li,Wenheng Liu,Shaohua Li,Hui Xin,Kai Tan,Zhexun LianPubMed 37915070
- Stem cells international 2023:79277472023Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Chenchen Zhou,Xiaobo Zhao,Ronghua Che,Yuelin Wu,Sheng Wan,Jinda Pei,Liping Yao,Xiaolin HuaPubMed 37559681
- Epigenetics 17:1738-17522022mA RNA demethylase FTO promotes the growth, migration and invasion of pancreatic cancer cells through inhibiting TFPI-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Ying He,Lu-Lu Zhai,Long-Jiang Chen,Li-Chao Yao,Lun Wu,Zhi-Gang Tang,Jin-Zhuo NingPubMed 35404184
- Frontiers in cell and developmental biology 9:7641092021Identification of the Recombinant Surface-Related Antigen as a Possible Immune Evasion Factor Against Human Splenic Fibroblasts by Targeting ITGB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaitian Fu,Jiachen Lu,Xinxin Zhang,Bo Wang,Yifan Sun,Yao Lei,Feihu Shen,Kokouvi Kassegne,Eun-Taek Han,Yang ChengPubMed 34938733
- Redox biology 46:1020842021PARP1 deficiency protects against hyperglycemia-induced neointimal hyperplasia by upregulating TFPI2 activity in diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao-Yang Wang,Meng-Qi Guo,Qing-Ke Cui,Haitao Yuan, Shan-Ji Fu,Bin Liu,Fei Xie,Wen Qiao,Jie Cheng,Ying Wang,Ming-Xiang ZhangPubMed 34364219
- Frontiers in oncology 11:6624342021TFPI2 Promotes Perivascular Migration in an Angiotropism Model of Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Mo,Xiulan Zhao,Wei Wang,Nan Zhao,Xueyi Dong,Yanhui Zhang,Runfen Cheng,Baocun SunPubMed 34249699
- Carcinogenesis 41:1767-17802020E2F5 promotes prostate cancer cell migration and invasion through regulation of TFPI2, MMP-2 and MMP-9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepmala Karmakar,Jyotirindra Maity,Payel Mondal,Puskar Shyam Chowdhury,Nilabja Sikdar,Parimal Karmakar,Chandrima Das,Sanghamitra SenguptaPubMed 32386317
- Scientific reports 10:40362020Indirect regulation of TFPI-2 expression by miR-494 in breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianne S Andresen,Benedicte Stavik,Marit Sletten,Mari Tinholt,Per Morten Sandset,Nina Iversen,Grethe SkrettingPubMed 32132611
- International journal of molecular sciences 19:2018Involvement of MAFB and MAFF in Retinoid-Mediated Suppression of Hepatocellular Carcinoma Invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyuki Tsuchiya et. al.PubMed 29757260
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 - 1/60 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
Alternative names
Tissue factor pathway inhibitor 2, TFPI-2, Placental protein 5, PP5, TFPI2
Recommended products
Rabbit Recombinant Monoclonal TFPI2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14442
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--western-blot-img146830.jpg "Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)")
Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
Blocking/ Dilution buffer: 5% NFDM /TBST.
All lanes: Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/5000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
![Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--western-blot-img57556.jpg "Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)")
Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
Blocking/ Dilution buffer: 5% NFDM /TBST.
All lanes: Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/10000 dilution
Lane 1: Rat spleen lysate at 10 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
![Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--western-blot-img104340.jpg "Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)")
Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
Blocking/ Dilution buffer: 5% NFDM /TBST.
All lanes: Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Mouse spleen lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
![Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (ab186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--immunoprecipitation-img44227.jpg "Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (ab186747)")
Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (ab186747)
Lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was immunoprecipitated with ab186747 at a 1/50 dilution. For the subsequent blot, ab186747 was used at a 1/1000 dilution, with a 1/1000 dilution of Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated as the secondary antibody. Blocking/ Dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (ab186747)
Predicted band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] (ab186747), expandable thumbnail](https://content.abcam.com/products/images/tfpi2-antibody-epr14442-ab186747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113689.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] (ab186747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] (ab186747)
Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TFPI2 using ab186747 at a 1/250 dilution. A prediluted HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com