AB246362

Anti-TFPI2 antibody [EPR14442] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal TFPI2 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Tissue factor pathway inhibitor 2, TFPI-2, Placental protein 5, PP5, TFPI2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (AB246362)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (AB246362)

Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TFPI2 using ab186747 at a 1/250 dilution. A prediluted HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab186747).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (AB246362)

Lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was immunoprecipitated with ab186747 at a 1/50 dilution. For the subsequent blot, ab186747 was used at a 1/1000 dilution, with a 1/1000 dilution of Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated as the secondary antibody. Blocking/ Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab186747).

All lanes:

Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (<a href='/en-us/products/primary-antibodies/tfpi2-antibody-epr14442-ab186747'>ab186747</a>)

Predicted band size: 27 kDa

false

Unconjugated

Anti-TFPI2 antibody [EPR14442]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14442

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

ab246362 is the carrier-free version of ab186747.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

See full target information TFPI2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 3:611 PubMed33097830

2020

Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Hou,Yuanyang Dong,Jingxi Huang,Chaoyong Liao,Jiaqi Lei,Youli Wang,Yujiao Lai,Yifei Bian,Yang He,Jingjing Sun,Meng Sun,Qiuyu Jiang,Bo Wang,Zhengquan Yu,Yuming Guo,Bingkun Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TFPI2 antibody [EPR14442] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (AB246362)

Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (AB246362)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise