Anti-TG6 antibody [EPR10308] - N-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

Immunohistochemical analysis of paraffin- embedded Human adenocarcinoma of endometrium tissue labeling TG6 with ab180959 at 1/100 dilution. Secondary antibody ImmunoHistoprobe HRP Polymer for Rabbit IgG pre-diluted.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling TG6 with ab180959 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing membranous staining in MOLT-4 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : PBS instead of the primary antibody.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 cells labelling TG6 with ab180959 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing membranous staining in RAW 264.7 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : PBS instead of the primary antibody.

• WB

Supplier Data

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

All lanes:

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/5000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa,33 kDa,34 kDa,39 kDa,79 kDa,93 kDa

Observed band size: 120 kDa,34 kDa,39 kDa,79 kDa,93 kDa

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• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed MOLT4 cells labelling TG6 (green) or negative control (red) using ab180959 at 1/20 dlution followed by Goat anti rabbit IgG (FITC) at 1/150 diltuion.

• WB

Lab

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

This antibody showed positive in mouse kidney, aorta and heart, weak in skeletal muscle and liver which is consistent with PMID : 22194042. And it detected signals were increased in the Neuro-2a differentiated group which is consistent with PMID : 21984379.

All lanes:

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/1000 dilution

Lane 1:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse aorta tissue lysate at 20 µg

Lane 6:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 7:

Mouse liver tissue lysate at 20 µg

Lane 8:

Undifferentiated Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 9:

Neuro-2a differentiated for 72 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

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Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

All lanes:

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/2000 dilution

All lanes:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 79 kDa

false

• WB

Lab

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (AB180959)

This data was generated using the same antibody clone in a different buffer formulation (ab180959).

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 70 s

All lanes:

Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate at 10 µg

Lane 2:

Human kidney lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

CTLL-2 (Mouse T lymphocyte) cell lysate at 10 µg

Lane 5:

EL4 (Mouse lymphoma T lymphocyte) cell lysate at 10 µg

Lane 6:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate at 10 µg

Lane 7:

NIH/3T3 (Mouse embryonic fibroblast) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 79 kDa

Observed band size: 75 kDa

false