Rabbit Polyclonal TGF alpha antibody. Suitable for ELISA, WB, sELISA, Neut and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TGFA.
Images
Publications
- Nature communications 14:15592023Cellular mechanisms of heterogeneity in NF2-mutant schwannoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristine Chiasson-MacKenzie,Jeremie Vitte,Ching-Hui Liu,Emily A Wright,Elizabeth A Flynn,Shannon L Stott,Marco Giovannini,Andrea I McClatcheyPubMed 36944680
- The Journal of clinical investigation 132:2022Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Cai,Ligen Shi,Jingyan Zhao,Fei Xu,Connor Dufort,Qing Ye,Tuo Yang,Xuejiao Dai,Junxuan Lyu,Chenghao Jin,Hongjian Pu,Fang Yu,Sulaiman Hassan,Zeyu Sun,Wenting Zhang,T Kevin Hitchens,Yejie Shi,Angus W Thomson,Rehana K Leak,Xiaoming Hu,Jun ChenPubMed 35912857
Key facts
- Isotype
Unknown
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TGFA. Database link P01135
Reactivity data
Select an application| ELISA | WB | sELISA | Neut | |
|---|---|---|---|---|
| Human | Expected | Expected | Predicted | Expected |
| Mouse | Predicted | Expected | Predicted | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required. |
Species Human | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Alternative names
Protransforming growth factor alpha, TGFA
Recommended products
Rabbit Polyclonal TGF alpha antibody. Suitable for ELISA, WB, sELISA, Neut and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TGFA.
Alternative names
Protransforming growth factor alpha, TGFA
Key facts
- Isotype
Unknown
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TGFA. Database link P01135
- Purification technique
Affinity purification Immunogen
- Specificity
The immunogen shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.
- Light chain type
unknown
- Reconstitution
Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-TGF alpha antibody [EPR15346] ab208156
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Sandwich ELISA - Anti-TGF alpha antibody (ab9585)
ab9585 detecting TGF-alpha using sELISA
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