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AB9585

Anti-TGF alpha antibody

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(21 Publications)

Rabbit Polyclonal TGF alpha antibody. Suitable for ELISA, WB, sELISA, FuncS (Neut/Block) and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Protransforming growth factor alpha.

View Alternative Names

Protransforming growth factor alpha, TGFA

1 Images
Sandwich ELISA - Anti-TGF alpha antibody (AB9585)
  • sELISA

Unknown

Sandwich ELISA - Anti-TGF alpha antibody (AB9585)

ab9585 detecting TGF-alpha using sELISA

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

No

Reacts with

Human, Mouse

Applications

sELISA, FuncS (Neut/Block), WB, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Protransforming growth factor alpha.

P01135

Specificity

The immunogen shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required.</p>" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required.</p>" }, "Recombinant full length protein - Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α.</p>", "FuncSNeutBlock-species-checked": "notRecommended", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "" } } }

Product details

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab208156

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
See full target information Protransforming growth factor alpha

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:1559 PubMed36944680

2023

Cellular mechanisms of heterogeneity in NF2-mutant schwannoma.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Chiasson-MacKenzie,Jeremie Vitte,Ching-Hui Liu,Emily A Wright,Elizabeth A Flynn,Shannon L Stott,Marco Giovannini,Andrea I McClatchey

The Journal of clinical investigation 132: PubMed35912857

2022

Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cai,Ligen Shi,Jingyan Zhao,Fei Xu,Connor Dufort,Qing Ye,Tuo Yang,Xuejiao Dai,Junxuan Lyu,Chenghao Jin,Hongjian Pu,Fang Yu,Sulaiman Hassan,Zeyu Sun,Wenting Zhang,T Kevin Hitchens,Yejie Shi,Angus W Thomson,Rehana K Leak,Xiaoming Hu,Jun Chen

Acta physiologica (Oxford, England) 235:e13828 PubMed35543087

2022

Increased COX-2 after ureter obstruction attenuates fibrosis and is associated with EP receptor upregulation in mouse and human kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Signe S Tofteng,Line Nilsson,Amalie K Mogensen,Rikke Nørregaard,Rolf Nüsing,Mikhail Diatchikhine,Lars Lund,Claus Bistrup,Boye L Jensen,Kirsten Madsen

Nature medicine 28:752-765 PubMed35411077

2022

Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Cátia Monteiro,Lauritz Miarka,María Perea-García,Neibla Priego,Pedro García-Gómez,Laura Álvaro-Espinosa,Ana de Pablos-Aragoneses,Natalia Yebra,Diana Retana,Patricia Baena,Coral Fustero-Torre,Osvaldo Graña-Castro,Kevin Troulé,Eduardo Caleiras,Patricia Tezanos,Pablo Muela,Elisa Cintado,José Luis Trejo,Juan Manuel Sepúlveda,Pedro González-León,Luis Jiménez-Roldán,Luis Miguel Moreno,Olga Esteban,Ángel Pérez-Núñez,Aurelio Hernández-Lain,José Mazarico Gallego,Irene Ferrer,Rocío Suárez,Eva M Garrido-Martín,Luis Paz-Ares,Celine Dalmasso,Elizabeth Cohen-Jonathan Moyal,Aurore Siegfried,Aisling Hegarty,Stephen Keelan,Damir Varešlija,Leonie S Young,Malte Mohme,Yvonne Goy,Harriet Wikman,Jose Fernández-Alén,Guillermo Blasco,Lucía Alcázar,Clara Cabañuz,Sergei I Grivennikov,Andrada Ianus,Noam Shemesh,Claudia C Faria,Rebecca Lee,Paul Lorigan,Emilie Le Rhun,Michael Weller,Riccardo Soffietti,Luca Bertero,Umberto Ricardi,Joaquim Bosch-Barrera,Elia Sais,Eduard Teixidor,Alejandro Hernández-Martínez,Alfonso Calvo,Javier Aristu,Santiago M Martin,Alvaro Gonzalez,Omer Adler,Neta Erez,Manuel Valiente

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 37:1012-1023 PubMed35191092

2022

EGFR Signaling Is Required for Maintaining Adult Cartilage Homeostasis and Attenuating Osteoarthritis Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Wei,Xiaoyuan Ma,Hao Sun,Tao Gui,Jun Li,Lutian Yao,Leilei Zhong,Wei Yu,Biao Han,Charles L Nelson,Lin Han,Frank Beier,Motomi Enomoto-Iwamoto,Jaimo Ahn,Ling Qin

Oncology letters 22:639 PubMed34386061

2021

Triple therapy with osimertinib, bevacizumab and cetuximab in EGFR-mutant lung cancer with HIF-1α/TGF-α expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuya Nishii,Kadoaki Ohashi,Hiromi Watanabe,Go Makimoto,Takamasa Nakasuka,Hisao Higo,Kiichiro Ninomiya,Yuka Kato,Toshio Kubo,Kammei Rai,Eiki Ichihara,Katsuyuki Hotta,Masahiro Tabata,Yoshinobu Maeda,Katsuyuki Kiura

Molecular biology of the cell 32:348-361 PubMed33378218

2020

Suppression of α-catenin and adherens junctions enhances epithelial cell proliferation and motility via TACE-mediated TGF-α autocrine/paracrine signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Eric N Bunker,Graycen E Wheeler,Douglas A Chapnick,Xuedong Liu

Molecular therapy. Nucleic acids 22:640-656 PubMed33230463

2020

TNFAIP8 Promotes Cisplatin Chemoresistance in Triple-Negative Breast Cancer by Repressing p53-Mediated miR-205-5p Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yu Ma,Yang Li,Hui-Zi Yin,Hang Yin,Yuan-Yuan Qu,Qing-Yong Xu

The Journal of surgical research 244:547-557 PubMed31351398

2019

Repair Effects of KGF on Ischemia-Reperfusion-Induced Flap Injury via Activating Nrf2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yueping Mao,Xiaoyan Chen,Yue Xia,Xiaoyuan Xie

BioMed research international 2019:8231267 PubMed30906781

2019

Blockage of TGF- Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

YiXun Li,Huan Li,Yong Duan,XueMei Cai,DingYun You,Feng Zhou,ChuanQi Yang,XiaoYu Tuo,ZiJie Liu
View all publications

Product promise

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