Anti-TGF beta 1 antibody [EPR18163]
- RabMAb
- Recombinant
- KO Validated
- What is this?
4
(3 Reviews)
|
(142 Publications)
Anti-TGF beta 1 antibody [EPR18163] (ab179695) is a rabbit monoclonal antibody detecting TGF beta 1 in Western Blot. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 100 publications
View Alternative Names
TGFB, TGFB1, Transforming growth factor beta-1 proprotein
- WB
Lab
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. For testing samples with low expression level of TGF beta 1, we recommend ab179695 which could give stronger signal. Loading larger amount of lysate or lower antibody dilution would also help.
Lanes 1 - 8:
Western blot - Anti-TGF beta 1 antibody [EPR21143] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-antibody-epr21143-ab215715'>ab215715</a>) at 1/1000 dilution
Lanes 1 - 8:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
Lane 1:
A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg
Lane 3:
K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lanes 4 - 5:
NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 6:
Mouse spleen tissue lysate
Lane 7:
Rat spleen tissue lysate
Lane 8:
Mouse heart tissue lysate
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa,12 kDa
false
Exposure time: 20s
- WB
Lab
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
Lanes 1 - 4 : Merged signal (red and green). Green - ab179695 observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab179695 was shown to react with TGF beta in wild-type A549 cells in Western blot with loss of signal observed in TGFB1 knockout cell line ab269509 (TGFB1 knockout cell lysate ab269671). Wild-type A549 and TGFB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab179695 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
Western blot - Human TGFB1 (TGF beta 1) knockout A549 cell lysate (<a href='/en-us/products/cell-lysates/human-tgfb1-tgf-beta-1-knockout-a549-cell-lysate-ab269671'>ab269671</a>) at 20 µg
Lane 2:
TGFB1 knockout A549 cell lysate at 20 µg
Lane 3:
K562 cell lysate at 20 µg
Lane 4:
SH-SY5Y cell lysate at 20 µg
Predicted band size: 44 kDa
Observed band size: 48 kDa
false
- WB
Supplier Data
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : Lane 1 : 1 second; Lane 2/3 : 3 minutes.
Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house.
All lanes:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1:
Recombinant protein fragment human TGF beta 1 at 0.01 µg
Lane 2:
Recombinant protein fragment human TGF beta 2 at 0.01 µg
Lane 3:
Recombinant protein fragment human TGF beta 3 at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 44 kDa
false
- WB
Supplier Data
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
Blocking/Dilution buffer : 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID : 18390240; PMID : 12717387; PMID : 2139036).
All lanes:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1:
Human kidney lysate at 10 µg
Lane 2:
Human spleen lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5 kDa,50 kDa
false
Exposure time: 3min
- WB
Lab
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
** Lanes 1 - 4 : ** Merged signal (red and green). Green - ab179695 observed at 50 kDa. Red - loading control, ab8245 observed at 37 kDa. ab179695 was shown to react with TGF beta 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255439 (knockout cell lysate ab263799) was used. Wild-type and TGF beta 1 knockout samples were subjected to SDS-PAGE. ab179695 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1:
A549 cell lysate at 20 µg
Lane 2:
K562 cell lysate at 20 µg
Lane 2:
Western blot - Human TGFB1 (TGF beta 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tgfb1-tgf-beta-1-knockout-hela-cell-line-ab255439'>ab255439</a>)
Lane 3:
Wild-type HeLa cell lysate at 20 µg
Lane 4:
TGF beta 1 knockout HeLa cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 37 kDa,50 kDa
false
- WB
Supplier Data
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : Lane 1 : 3 minutes; Lane 2-4 : 30 seconds.
All lanes:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution
Lane 1:
L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate at 10 µg
Lane 2:
RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3:
A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg
Lane 4:
K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
false
- WB
Supplier Data
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : Lane 1/2/3/5 : 3minutes; Lane 4 : 30 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID : 18390240; PMID : 12717387; PMID : 2139036).
Lane 1:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution
Lanes 2 - 5:
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1:
Mouse serum at 10 µg
Lane 2:
Mouse spleen lysate at 10 µg
Lane 3:
Rat kidney lysate at 10 µg
Lane 4:
Rat spleen lysate at 10 µg
Lane 5:
Rat serum at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5 kDa,50 kDa
false
- WB
CiteAb
Western blot - Anti-TGF beta 1 antibody [EPR18163] (AB179695)
TGF beta 1 western blot using anti-TGF beta 1 antibody [EPR18163] ab179695. Publication image and figure legend from Song, L., Qu, D., et al., 2017, Sci Rep, PubMed 28169366.
ab179695 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab179695 please see the product overview.
The liver pathologies in the different groups of NAFLD rats.(A) Hematoxylin and eosin (HE) staining (200×) and hepatic steatosis scores. (B) Oil Red O staining of liver tissues (200×). The NC, HF, PSEL, PSEM and PSEH groups are described in Fig. 1. The hepatic steatosis degree was decreased by PSEs intervention compared with that in the HF group.
false
Related conjugates and formulations (1)
-
Anti-TGF beta 1 antibody [EPR18163] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-TGF beta 1 antibody [EPR18163] (ab179695) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of TGF beta 1?
Anti-TGF beta 1 [EPR18163] (ab179695) specifically detects a band for TGF beta 1 (UniProt: P04202) at a molecular weight of 44kDa.
Trusted by the scientific community
Anti-TGF beta 1 [EPR18163] (ab179695) was first used in a scientific publication in 2016 and has been cited over 100 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-TGF beta 1 antibody [EPR18163] (ab179695) has been confirmed by Western blot testing in TGFB1 Knockout HeLa cell line, ab255439.
Other related products
We have a range of other formats of antibody clone [EPR18163] also available for your convenience: ab179695, Carrier free - ab233730
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
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- Visit the General protocols
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Target data
Publications (142)
Recent publications for all applications. Explore the full list and refine your search
Journal of nanobiotechnology 23:648 PubMed41074162
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Science advances 11:eadu8422 PubMed40561034
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iScience 28:112517 PubMed40491477
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Materials today. Bio 32:101854 PubMed40487180
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Journal of clinical and translational hepatology 13:456-468 PubMed40474887
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Acta pharmaceutica Sinica. B 15:1643-1658 PubMed40370563
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Scientific reports 15:8260 PubMed40064976
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Toxicology and applied pharmacology 496:117260 PubMed39929281
2025
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Scientific reports 15:3619 PubMed39880976
2025
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