AB251436

Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal TGF beta 1 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

TGFB, TGFB1, Transforming growth factor beta-1 proprotein

4 Images

Supplier Data

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

This data was developed using ab205604, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-3 : 30 seconds; Lane 4 : 3 minutes.

All lanes:

Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-tgf-beta-3-antibody-epr19867-ab205604'>ab205604</a>) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 10 µg

Lane 3:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Lane 4:

L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa

Observed band size: 12.5 kDa,44 kDa

false

Unknown

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

This data was developed using ab205604, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The cleaved form observed is consistent with what has been described in the literature (PMID : 12717387; PMID : 2139036; PMID : 3208746).

Lane 1:

Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-tgf-beta-3-antibody-epr19867-ab205604'>ab205604</a>) at 1/5000 dilution

Lanes 2 - 3:

Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-tgf-beta-3-antibody-epr19867-ab205604'>ab205604</a>) at 1/1000 dilution

Lane 1:

Human fetal spleen tissue lysate at 10 µg

Lane 2:

Rat spleen tissue lysate at 10 µg

Lane 3:

Mouse spleen tissue lysate at 10 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Lanes 2 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 44 kDa

Observed band size: 12.5 kDa,44 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

This data was developed using ab205604, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 3 minutes; Lane 3 : 15 seconds.

Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house.

Lane 1:

Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-tgf-beta-3-antibody-epr19867-ab205604'>ab205604</a>) at 1/10000 dilution

Lanes 2 - 3:

Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-tgf-beta-3-antibody-epr19867-ab205604'>ab205604</a>) at 1/1000 dilution

Lane 1:

Recombinant protein fragment human TGF beta 1 at 0.01 µg

Lane 2:

Recombinant protein fragment human TGF beta 2 at 0.01 µg

Lane 3:

Recombinant protein fragment human TGF beta 3 at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

This data was developed using ab205604, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The cleaved form observed is consistent with what has been described in the literature (PMID : 12717387; PMID : 2139036; PMID : 3208746).

All lanes:

Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-tgf-beta-3-antibody-epr19867-ab205604'>ab205604</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa,58 kDa

Observed band size: 12.5 kDa,44 kDa,55 kDa

false

Exposure time: 1min

Unconjugated

Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19867

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

ab251436 is the carrier-free version of ab205604.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transforming growth factor beta-1 proprotein : Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.. Latency-associated peptide. Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix (PubMed : 28117447). Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1 (PubMed : 19651619, PubMed : 19750484, PubMed : 2022183, PubMed : 22278742, PubMed : 8617200, PubMed : 8939931). Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs) (PubMed : 19651619, PubMed : 19750484, PubMed : 22278742). Interaction with integrins (ITGAV : ITGB6 or ITGAV : ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1 (PubMed : 22278742, PubMed : 28117447).. Transforming growth factor beta-1. Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration (By similarity). Activation into mature form follows different steps : following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix (PubMed : 29109152). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus (PubMed : 19651619, PubMed : 19750484, PubMed : 2022183, PubMed : 22278742, PubMed : 8617200, PubMed : 8939931). TGF-beta-1 is released from LAP by integrins (ITGAV : ITGB6 or ITGAV : ITGB8) : integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1 (PubMed : 22278742, PubMed : 28117447). Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (PubMed : 20207738). While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules' (By similarity). Plays an important role in bone remodeling : acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner (By similarity). At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development (By similarity). At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (PubMed : 25310401). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus (PubMed : 25893292, PubMed : 29483653, PubMed : 30696809). Positively regulates odontoblastic differentiation in dental papilla cells, via promotion of IPO7-mediated translocation of phosphorylated SMAD2 to the nucleus and subsequent transcription of target genes (By similarity). Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (PubMed : 25893292, PubMed : 30696809).

See full target information TGFB1

Additional targets

TGFB3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

Western blot - Anti-TGF beta 1+TGF beta 3 antibody [EPR19867] - BSA and Azide free (AB251436)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (1)

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise