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Rabbit Recombinant Monoclonal TGF beta 2 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

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Images

Sandwich ELISA - Anti-TGF beta 2 antibody [EPR12-185] - BSA and Azide free Detector (AB323676), expandable thumbnail
  • Sandwich ELISA - Anti-TGF beta 2 antibody [EPR12-185] - BSA and Azide free Detector (AB323676), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Transforming growth factor beta-2 proprotein. Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide. Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2. Multifunctional protein that regulates various processes such as angiogenesis and heart development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).

Alternative names

Transforming growth factor beta-2 proprotein, Tgfb2

Rabbit Recombinant Monoclonal TGF beta 2 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EUG-12-SBC-185
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab323676 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse TGF beta 2. The recommended pair for sandwich ELISA is:

Capture: Anti-TGF beta 2 antibody [EPR12-110] - BSA and Azide free Capture ab323675, Mouse TGF beta 2 Capture Antibody (unconjugated)

Detector: ab323676, Mouse TGF beta 2 Detector Antibody (unconjugated)

The reference range value is 97 - 6250 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Anti-TGF beta 2 antibody [EPR12-185] - BSA and Azide free Detector (ab323676), expandable thumbnail

    Sandwich ELISA - Anti-TGF beta 2 antibody [EPR12-185] - BSA and Azide free Detector (ab323676)

    Sandwich ELISA using mouse total TGF-beta 2 immunogen protein standard curve with the capture antibody Anti-TGF beta 2 antibody [EPR12-110] - BSA and Azide free Capture ab323675 at 2 ug/mL and detector antibody ab323676 at 0.5 ug/mL. Background-subtracted absorbance data values (mean +/- standard deviation) are displayed.

  • Sandwich ELISA - Anti-TGF beta 2 antibody [EPR12-185] - BSA and Azide free Detector (ab323676), expandable thumbnail

    Sandwich ELISA - Anti-TGF beta 2 antibody [EPR12-185] - BSA and Azide free Detector (ab323676)

    Interpolated concentration of total TGF-beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: neat serum and plasma (EDTA). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com