Anti-TGF beta 3 antibody
4
(5 Reviews)
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(62 Publications)
Anti-TGF beta 3 antibody (ab15537) is a rabbit polyclonal antibody detecting TGF beta 3 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rabbit, Rat.
- Over 50 publications
- Trusted since 2004
View Alternative Names
Transforming growth factor beta-3 proprotein, TGFB3
- WB
Unknown
Western blot - Anti-TGF beta 3 antibody (AB15537)
All lanes:
Western blot - Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution
All lanes:
Western blot - Recombinant human TGF beta 3 protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-tgf-beta-3-protein-active-ab52313'>ab52313</a>) at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 47 kDa
true
Exposure time: 30s
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (AB15537)
Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-TGF beta 3 antibody (AB15537)
ICC/IF image of ab15537 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5μg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
- IHC-P
AbReview8981****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (AB15537)
ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Reactivity data
Product details
Anti-TGF beta 3 antibody (ab15537) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rabbit, Rat samples.
What is the molecular weight of TGF beta 3?
Anti-TGF beta 3 (ab15537) specifically detects a band for TGF beta 3 (UniProt: P10600) at a molecular weight of 47kDa.
Trusted by the scientific community
Anti-TGF beta 3 (ab15537) was first used in a scientific publication in 2004 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-TGF beta 3 (ab15537) has over 5 independent reviews from customers.
Properties and storage information
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Purification technique
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Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
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Target data
Publications (62)
Recent publications for all applications. Explore the full list and refine your search
Life (Basel, Switzerland) 15: PubMed41010372
2025
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NPJ Regenerative medicine 10:4 PubMed39833274
2025
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BMC biology 22:193 PubMed39256768
2024
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Journal of translational medicine 22:653 PubMed39004699
2024
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Regenerative therapy 26:180-187 PubMed38948131
2024
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JCI insight 9: PubMed38175727
2024
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Journal of biochemical and molecular toxicology 38:e23581 PubMed38044485
2023
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Cureus 15:e42412 PubMed37637625
2023
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Cardiovascular research 119:2061-2073 PubMed37200403
2023
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BMC musculoskeletal disorders 24:325 PubMed37098516
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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