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AB15537

Anti-TGF beta 3 antibody

4

(5 Reviews)

|

(62 Publications)

Anti-TGF beta 3 antibody (ab15537) is a rabbit polyclonal antibody detecting TGF beta 3 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rabbit, Rat.

- Over 50 publications
- Trusted since 2004

View Alternative Names

Transforming growth factor beta-3 proprotein, TGFB3

4 Images
Western blot - Anti-TGF beta 3 antibody (AB15537)
  • WB

Unknown

Western blot - Anti-TGF beta 3 antibody (AB15537)

All lanes:

Western blot - Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution

All lanes:

Western blot - Recombinant human TGF beta 3 protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-tgf-beta-3-protein-active-ab52313'>ab52313</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 47 kDa

true

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (AB15537)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (AB15537)

Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).

Immunocytochemistry/ Immunofluorescence - Anti-TGF beta 3 antibody (AB15537)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TGF beta 3 antibody (AB15537)

ICC/IF image of ab15537 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5μg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (AB15537)
  • IHC-P

AbReview8981****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (AB15537)

ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rabbit, Mouse, Rat

Applications

IP, IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. ab15537 demonstrates cytoplasmic staining.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. ab15537 demonstrates cytoplasmic staining.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. ab15537 demonstrates cytoplasmic staining.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rabbit": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. ab15537 demonstrates cytoplasmic staining.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

Product details

What is this antibody validated in?
Anti-TGF beta 3 antibody (ab15537) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rabbit, Rat samples.

What is the molecular weight of TGF beta 3?
Anti-TGF beta 3 (ab15537) specifically detects a band for TGF beta 3 (UniProt: P10600) at a molecular weight of 47kDa.

Trusted by the scientific community
Anti-TGF beta 3 (ab15537) was first used in a scientific publication in 2004 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-TGF beta 3 (ab15537) has over 5 independent reviews from customers.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transforming growth factor beta-3 proprotein : Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.. Latency-associated peptide. Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3 (By similarity). Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3 (By similarity).. Transforming growth factor beta-3 : Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity). Activation into mature form follows different steps : following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus (By similarity). TGF-beta-3 is released from LAP by integrins : integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3 (By similarity). Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
See full target information TGFB3

Publications (62)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 15: PubMed41010372

2025

A Dose-Dependent Study Examining Dexmedetomidine's Possible Effects Against Oxidative, Fibrotic, and Apoptotic Damage Induced by Radiation Exposure in Spleen Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Hatice Beyazal Polat,Hamit Yılmaz,Kagan Kilinc,Belemir Gülhan,Sema Yılmaz Rakıcı,Levent Tümkaya

NPJ Regenerative medicine 10:4 PubMed39833274

2025

Systemic factors associated with antler growth promote complete wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Guo,Guokun Zhang,Jing Ren,Jiping Li,Zhen Wang,Hengxing Ba,Zihao Ye,Ying Wang,Junjun Zheng,Chunyi Li

BMC biology 22:193 PubMed39256768

2024

Hypoxia and Foxn1 alter the proteomic signature of dermal fibroblasts to redirect scarless wound healing to scar-forming skin wound healing in Foxn1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Gawronska-Kozak,Sylwia Machcinska-Zielinska,Katarzyna Walendzik,Marta Kopcewicz,Mirva Pääkkönen,Joanna Wisniewska

Journal of translational medicine 22:653 PubMed39004699

2024

EGFR mutations induce the suppression of CD8 T cell and anti-PD-1 resistance via ERK1/2-p90RSK-TGF-β axis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huayan Huang,Xiaokuan Zhu,Yongfeng Yu,Ziming Li,Yi Yang,Liliang Xia,Shun Lu

Regenerative therapy 26:180-187 PubMed38948131

2024

Skeletal muscle injury treatment using the Silk Elastin® injection in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Kyohei Nakata,Masakazu Ishikawa,Naosuke Kamei,Shigeru Miyaki,Nobuo Adachi,Keiichiro Inoue,Shingo Kawabata

JCI insight 9: PubMed38175727

2024

The super-healing MRL strain promotes muscle growth in muscular dystrophy through a regenerative extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph G O'Brien,Alexander B Willis,Ashlee M Long,Jason Kwon,GaHyun Lee,Frank W Li,Patrick Gt Page,Andy H Vo,Michele Hadhazy,Melissa J Spencer,Rachelle H Crosbie,Alexis R Demonbreun,Elizabeth M McNally

Journal of biochemical and molecular toxicology 38:e23581 PubMed38044485

2023

Mechanistic study on ursolic acid inhibiting the growth of colorectal cancer cells through the downregulation of TGF-β3 by miR-140-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Fenfen Xiang,Xiaoxiao Li,Zixi Chen,Jun Wang,Jiahui Guo,Shanshan Zhu,Jun Zhou,Xiangdong Kang,Rong Wu

Cureus 15:e42412 PubMed37637625

2023

Interplay of Transforming Growth Factor-Beta 1 and 3 in the Pathogenesis of Oral Submucous Fibrosis and Its Malignant Transformation: An Immunohistochemical Study.

Applications

Unspecified application

Species

Unspecified reactive species

Shivani Bansal,Treville Pereira,Rajiv S Desai,Abinashi Jena,Poorvashree P Bobade,Madhura Patil

Cardiovascular research 119:2061-2073 PubMed37200403

2023

Transforming growth factor-β2 is associated with atherosclerotic plaque stability and lower risk for cardiovascular events.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Edsfeldt,Pratibha Singh,Frank Matthes,Christoffer Tengryd,Michele Cavalera,Eva Bengtsson,Pontus Dunér,Petr Volkov,Glykeria Karadimou,Anton Gisterå,Marju Orho-Melander,Jan Nilsson,Jiangming Sun,Isabel Gonçalves

BMC musculoskeletal disorders 24:325 PubMed37098516

2023

Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ming Wu,Yun-Jiao Wang,Shuai-Feng Li,Jing-Kun Wang,Jun Liu,Chao-Chao Fan,Yan Xiong
View all publications

Product promise

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