AB121024

Anti-TGF beta Receptor I antibody

Name: Anti-TGF beta Receptor I antibody. Goat polyclonal (ab121024) | Abcam
Brand: Abcam Limited
SKU: AB121024
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(11 Publications)

Goat Polyclonal TGF beta Receptor I antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human TGFBR1 aa 50-100.

View Alternative Names

ALK5, SKR4, TGFBR1, TGF-beta receptor type-1, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, Serine/threonine-protein kinase receptor R4, TGF-beta type I receptor, Transforming growth factor-beta receptor type I, ALK-5, TGF-beta receptor type I, TbetaR-I

2 Images

Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (AB121024)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (AB121024)

Flow cytometric (Intracellular) analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supplier Data

Western blot - Anti-TGF beta Receptor I antibody (AB121024)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

Lane 1:

Western blot - Anti-TGF beta Receptor I antibody (ab121024) at 0.1 µg/mL

Lane 2:

Western blot - Anti-TGF beta Receptor I antibody (ab121024) at 0.03 µg/mL

Lane 1:

Rat liver lysate in RIPA buffer at 35 µg

Lane 2:

Rat lung lysate in RIPA buffer at 35 µg

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human TGFBR1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P36897

Specificity

ab121024 is expected to recognize both reported isoforms (NP_004603.1; NP_001124388.1). The immunizing peptide represents part of a potential extracellular domain.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab121024 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.

See full target information TGFBR1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Research (Washington, D.C.) 8:0619 PubMed39975575

2025

Regulating Integrin β1 to Restore Gonadotropin-Releasing Hormone-Tanycyte Unit Function in Polycystic Ovary Syndrome-Related Hypothalamic Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Xiaoyu Tong,Yan Xiao,Yicong Wang,Wei Hu,Wenhan Lu,Yuning Chen,Jiajia Li,Wenhao Gao,Hongru Gao,Yicheng Tian,Sizhe Dai,Yi Feng

Chinese medicine 20:18 PubMed39910658

2025

Distinct mechanisms of electroacupuncture and manual acupuncture in modulating hypothalamic GnRH-tanycyte unit function of polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Yicong Wang,Yuning Chen,Wenhan Lu,Xiaoyu Tong,Jiajia Li,Wenhao Gao,Rui Huang,Wei Hu,Yi Feng

International journal of molecular sciences 25: PubMed38928268

2024

Amelioration of Fibrosis via S1P Inhibition Is Regulated by Inactivation of TGF-β and SPL Pathways in the Human Cornea.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Nicholas,Sandip K Basu,Nawajes Mandal,Dimitrios Karamichos

Bioengineered 13:930-940 PubMed34967706

2021

Long non-coding RNA myocardial infarction-associated transcript promotes 1-Methyl-4-phenylpyridinium ion-induced neuronal inflammation and oxidative stress in Parkinson's disease through regulating microRNA-221-3p/ transforming growth factor /nuclear factor E2-related factor 2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Lang,Hui Zhang,Haojia Yu,Yu Li,Xiao Liu,Minjie Li

Journal of cellular and molecular medicine 25:3498-3510 PubMed33682288

2021

TGF-β isoforms inhibit hepatitis C virus propagation in transforming growth factor beta/SMAD protein signalling pathway dependent and independent manners.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Li Zou,Jian-Rui Li,Hu Li,Jia-Li Tan,Mei-Xi Wang,Nan-Nan Liu,Rong-Mei Gao,Hai-Yan Yan,Xue-Kai Wang,Biao Dong,Yu-Huan Li,Zong-Gen Peng

International journal of biological macromolecules 165:1755-1764 PubMed33068624

2020

TMT-based quantitative proteomic analysis of antitumor mechanism of Sporisorium reilianum polysaccharide WM-NP-60 against HCT116 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lianbao Kan,Daizong Cui,Yangyang Chai,Ling Ma,Xiaoyan Li,Min Zhao

Medical science monitor : international medical jo 26:e922029 PubMed32447340

2020

Long Non-Coding RNA (LncRNA)-ATB Promotes Inflammation, Cell Apoptosis and Senescence in Transforming Growth Factor-β1 (TGF-β1) Induced Human Kidney 2 (HK-2) Cells via TGFβ/SMAD2/3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Han Sun,Cong Ke,Lin Zhang,Changjun Tian,Zhihui Zhang,Shuhua Wu

PloS one 14:e0220795 PubMed31437169

2019

Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mitsuru Seki,Nozomi Furukawa,Norimichi Koitabashi,Masaru Obokata,Simon J Conway,Hirokazu Arakawa,Masahiko Kurabayashi

Phytotherapy research : PTR 33:214-223 PubMed30375049

2018

(+)-Isobicyclogermacrenal and spathulenol from Aristolochia yunnanensis alleviate cardiac fibrosis by inhibiting transforming growth factor β/small mother against decapentaplegic signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lan-Lan Lou,Wei Li,Bin-Hua Zhou,Lin Chen,Han-Zhuang Weng,Yi-Hong Zou,Gui-Hua Tang,Xian-Zhang Bu,Sheng Yin

Scientific reports 8:1512 PubMed29367647

2018

Proteomic Analysis of Nucleus Pulposus Cell-derived Extracellular Matrix Niche and Its Effect on Phenotypic Alteration of Dermal Fibroblasts.

Applications

IHC-P

Species

Rabbit

Minting Yuan,Pei-Jing Pai,Xiaofen Liu,Henry Lam,Barbara P Chan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TGF beta Receptor I antibody

Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (AB121024)

Western blot - Anti-TGF beta Receptor I antibody (AB121024)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

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