Rabbit Recombinant Monoclonal TGF beta Receptor I antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples.
Images
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img171134.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303)")
![Immunoprecipitation - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--immunoprecipitation-img164501.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303)")
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img167845.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303)")
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img164595.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303)")
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img181401.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (AB288303)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Transfected cell lysate | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate | Dilution info - | Notes - |
Target data
Function
Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.
Alternative names
ALK5, SKR4, TGFBR1, TGF-beta receptor type-1, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, Serine/threonine-protein kinase receptor R4, TGF-beta type I receptor, Transforming growth factor-beta receptor type I, ALK-5, TGF-beta receptor type I, TbetaR-I
Recommended products
Rabbit Recombinant Monoclonal TGF beta Receptor I antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20923-13
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
This is the carrier-free version of Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img171134.jpg "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)")
Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)
False colour image of Western blot: Anti-TGF beta Receptor I antibody [EPR20923-13] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578 was shown to bind specifically to TGF beta Receptor I. A band was observed at 40/55 kDa in wild-type A549 cell lysates with no signal observed at this size in TGFBR1 knockout cell line Human TGFBR1 knockout A549 cell line ab277894 (knockout cell lysate ab283082). To generate this image, wild-type and TGFBR1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
This data was developed using Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] (Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: TGFBR1 knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human TGFBR1 knockout A549 cell line (Human TGFBR1 knockout A549 cell line ab277894)
Lane 3: A431 cell lysate at 20 µg
Lane 4: MOLT-4 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 40 kDa, 55 kDa
![Immunoprecipitation - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--immunoprecipitation-img164501.jpg "Immunoprecipitation - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)")
Immunoprecipitation - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)
TGF beta Receptor I was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578 in MCF7 whole cell lysate
The bands beneath the target band is caused by degradation as demonstrated by WB data.
This data was developed using Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578, the same antibody clone in a different buffer formulation.
All lanes: Immunoprecipitation - Anti-TGF beta Receptor I antibody [EPR20923-13] (Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578)
Predicted band size: 55 kDa
Observed band size: 55 kDa
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img167845.jpg "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)")
Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)
This data was developed using Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lane1 Lysate was made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: 81 seconds
All lanes: Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] (Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578) at 1/1000 dilution
Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell fresh lysate at 20 µg
Lane 2: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 37 kDa, 55 kDa
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img164595.jpg "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)")
Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)
This data was developed using Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysate were made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: 3 minutes
All lanes: Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] (Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 3: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 4: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
Lane 5: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 37 kDa, 55 kDa
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303), expandable thumbnail](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-bsa-and-azide-free-ab288303--western-blot-img181401.jpg "Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)")
Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] - BSA and Azide free (ab288303)
This data was developed using Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration:
Exposure time: 3 minutes
All lanes: Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] (Anti-TGF beta Receptor I antibody [EPR20923-13] ab235578) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: MCF7 transfected with 25nM siRNA specifically targeti TGF receptor I whole cell lysate at 20 µg
Lane 3: MCF7 transfected with 100nM siRNA specifically targeti TGF receptor I whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
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