Anti-TGF beta Receptor II antibody [EPR14673]

• WB

Lab

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (AB184948)

Lanes 1 - 4 : Merged signal (red and green). Green - ab184948 observed at 80 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab184948 was shown to specifically react with in wild-type A549 cells as signal was lost in TGFBR2 knockout cell line ab261863 (knockout cell lysate ab261672). Wild-type and TGFBR2 knockout samples were subjected to SDS-PAGE. ab184948 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/2000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TGFBR2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-tgfbr2-tgf-beta-receptor-ii-knockout-a549-cell-line-ab261863'>ab261863</a>)

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HT-1080 whole cell lysate at 20 µg

Predicted band size: 65 kDa

Observed band size: 80 kDa

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• WB

Supplier Data

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (AB184948)

All lanes:

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/5000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 65 kDa

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