Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (AB270609)

This data was developed using ab270440, the same antibody clone in a different buffer formulation.

A549 wild-type and TGF beta Receptor II knockout cells (ab261863) were labelled with a green or violet, fluorescent dye, respectively. Wild-type and knockout cells were mixed in a 1 : 1 ratio, fixed in 4% PFA and permeabilized in 0.1% Triton X-100.

Antibody staining was quantified using the Attune NxT Flow Cytometer with representative images showing the staining intensity in the knockout population (pink histogram, dashed line) compared to the wild-type cells (green histogram, solid line). Histograms with dotted lines represent secondary antibody-only controls in both wild-type and knockout cells.

Antibody dilution : 1/584

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (AB270609)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HT-1080 (Human fibrosarcoma cell line) cells labelling TGF beta receptor II with ab270440 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in HT-1080 cell line. Negative control : MCF-7 (Human breast adenocarcinoma cell line). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270440).

• Flow Cyt

Unknown

Flow Cytometry - Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (AB270609)

Flow cytometric analysis of permeabilized MCF7 (Human breast adenocarcinoma epithelial cell - Left) and HT-1080 (Human fibrosarcoma cell - Right) cells labelling TGF beta receptor II with ab270440 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control : MCF-7 cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270440).