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Rabbit Recombinant Monoclonal TGF beta Receptor II antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (AB259360), expandable thumbnail
  • Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (AB259360), expandable thumbnail
  • Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (AB259360), expandable thumbnail
  • Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (AB259360), expandable thumbnail
  • Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (AB259360), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and thus regulates a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. Isoform 1. Has transforming growth factor beta-activated receptor activity. Isoform 2. Has transforming growth factor beta-activated receptor activity. Isoform 3. Binds TGFB1, TGFB2 and TGFB3 in the picomolar affinity range without the participation of additional receptors. Blocks activation of SMAD2 and SMAD3 by TGFB1.

Alternative names

TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TbetaR-II, TGFBR2

Recommended products

Rabbit Recombinant Monoclonal TGF beta Receptor II antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24349-124
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360), expandable thumbnail

    Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:23468914).

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: Lanes 1-2: 81 seconds; Lane 3: 3 minutes.

    All lanes: Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360) at 1/1000 dilution

    Lane 1: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

    Observed band size: 70-80 kDa

  • Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360), expandable thumbnail

    Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MCF7, PC-12 (PMID:1310899).

    Lysates used in this blot have experienced freeze-thaw cycles. Bands below 50 kDa are caused by degradation.

    Exposure time: Lanes 1-4: 81 seconds; Lane 5: 3 minutes.

    All lanes: Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360) at 1/1000 dilution

    Lane 1: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 5: Rat lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

    Observed band size: 70-80 kDa

  • Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360), expandable thumbnail

    Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    TGF beta Receptor II was immunoprecipitated from 0.35 mg HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate 10 μg with ab259360 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259360 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate 10 μg

    Lane 2: ab259360 IP in HT-1080 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259360 in HT-1080 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds.

    Bands below 50 kDa are caused by degradation as demonstrated by WB data.

    All lanes: Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    Predicted band size: 65 kDa

    Observed band size: 70-80 kDa

  • Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360), expandable thumbnail

    Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Neuro-2a (PMID:1310899).

    Lysates used in this blot have experienced freeze-thaw cycles. Bands below 50 kDa are caused by degradation.

    Exposure time: 81 seconds.

    All lanes: Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 3: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

    Observed band size: 70-80 kDa

  • Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360), expandable thumbnail

    Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    TGF beta Receptor II was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg with ab259360 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259360 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg

    Lane 2: ab259360 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259360 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds.

    Bands below 50 kDa are caused by degradation as demonstrated by WB data.

    All lanes: Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    Predicted band size: 65 kDa

    Observed band size: 70-80 kDa

  • Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360), expandable thumbnail

    Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360)

    Western blot: Anti-TGFBR2 antibody [EPR24349-124] (ab259360) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab259360 was shown to bind specifically to TGFBR2. A band was observed at 65 kDa in wild-type A549 cell lysates with no signal observed at this size in TGFBR2 knockout cell line. To generate this image, wild-type and TGFBR2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-TGF beta Receptor II antibody [EPR24349-124] (ab259360) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 40 µg

    Lane 2: TGFBR2 knockout A549 cell lysate at 40 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 65 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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