AB183037

Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198]

Name: Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] (ab183037) | Abcam
Brand: Abcam Limited
SKU: AB183037
Price: 560 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal TGF beta Receptor II phospho S225 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TbetaR-II, TGFBR2

1 Images

Supplier Data

Western blot - Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] (AB183037)

All lanes:

Western blot - Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] (ab183037) at 1/10000 dilution

Lane 1:

Lysate from HepG2 cells treated with TGF-β at 10 µg

Lane 2:

Lysate from HepG2 cells treated with TGF-β then treated with alkaline phosphatase at 10 µg

Lane 3:

Lysate from HepG2 cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 65 kDa

Observed band size: 70-80 kDa

false

Carrier free

Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12198

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and thus regulates a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.. Isoform 1. Has transforming growth factor beta-activated receptor activity.. Isoform 2. Has transforming growth factor beta-activated receptor activity.. Isoform 3. Binds TGFB1, TGFB2 and TGFB3 in the picomolar affinity range without the participation of additional receptors. Blocks activation of SMAD2 and SMAD3 by TGFB1.

See full target information TGFBR2 phospho S225

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:201 PubMed40264229

2025

Sildenafil promotes osteogenic differentiation of human mesenchymal stem cells and inhibits bone loss by affecting the TGF-β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Menglong Hu,Likun Wu,Erfan Wei,Xingtong Pan,Qiyue Zhu,Xv Xiuyun,Letian Lv,Xinyi Dong,Hao Liu,Yunsong Liu

iScience 28:111756 PubMed39925433

2025

Negative regulation of lymphangiogenesis by Tenascin-C delays the resolution of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Katoh,Yoshiyuki Senga,Kento Mizutani,Kazuaki Maruyama,Daishi Yamakawa,Tadashi Yamamuro,Michiaki Hiroe,Keiichi Yamanaka,Akihiro Sudo,Naoyuki Katayama,Toshimichi Yoshida,Kyoko Imanaka-Yoshida

Oncogene 42:3633-3647 PubMed37864034

2023

Inhibition of TGF-β signaling, invasion, and growth of cutaneous squamous cell carcinoma by PLX8394.

Applications

Unspecified application

Species

Unspecified reactive species

Elina Siljamäki,Pilvi Riihilä,Ujjwal Suwal,Liisa Nissinen,Pekka Rappu,Markku Kallajoki,Veli-Matti Kähäri,Jyrki Heino

Journal of cellular physiology 237:1780-1789 PubMed34806177

2021

Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Chia Cheng,Chih-Feng Lin,Yong-Chong Lin,Tai-Horng Young,Pei-Jen Lou

OncoTargets and therapy 14:1487-1499 PubMed33679133

2021

FAST1 Predicts Poor Survival of Renal Carcinoma and Promotes Its Progression Through the TGF-β/Smad Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Tian,Xiangyang Fu,Liangliang Hu,Xiaofeng Yang,Peng Sun,Fengfeng Sun

Pathobiology : journal of immunopathology, molecul 87:100-113 PubMed31896114

2020

Growth Differentiation Factor 15 Promotes Progression of Esophageal Squamous Cell Carcinoma via TGF-β Type II Receptor Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Maiko Okamoto,Yu-Ichiro Koma,Takayuki Kodama,Mari Nishio,Manabu Shigeoka,Hiroshi Yokozaki

Oncogene 38:4637-4654 PubMed30742067

2019

MSC-regulated lncRNA MACC1-AS1 promotes stemness and chemoresistance through fatty acid oxidation in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wanming He,Bishan Liang,Chunlin Wang,Shaowei Li,Yang Zhao,Qiong Huang,Zexian Liu,Zhiqi Yao,Qijing Wu,Wangjun Liao,Shuyi Zhang,Yajing Liu,Yi Xiang,Jia Liu,Min Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198]

Western blot - Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] (AB183037)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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