AB99562

Anti-TGFBI antibody

Name: Anti-TGFBI antibody (ab99562) | Abcam
Brand: Abcam Limited
SKU: AB99562
Price: 535 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal TGFBI antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TGFBI aa 100-150.

View Alternative Names

BIGH3, TGFBI, Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody (AB99562)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody (AB99562)

ab99562 at 3.8μg/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody (AB99562)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody (AB99562)

Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Incubated with the primary antibody (ab99562) for 1hr at 10μg/ml followed by Alexa Fluor^® 488 secondary antibody at 2μg/ml. Shows golgi and membrane staining. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor^® 488 secondary antibody (2μg/ml).

Supplier Data

Western blot - Anti-TGFBI antibody (AB99562)

Lanes 1 - 2:

Western blot - Anti-TGFBI antibody (ab99562) at 0.3 µg/mL

Lane 3:

Western blot - Anti-TGFBI antibody (ab99562) at 0.1 mg/mL

Lane 1:

Human Kidney in RIPA buffer at 35 µg

Lane 2:

Colorectal cancer in RIPA buffer at 35 µg

Lane 3:

Human Prostate in RIPA buffer at 35 µg

Predicted band size: 75 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TGFBI aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q15582

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in cell adhesion (PubMed : 8024701). May play a role in cell-collagen interactions (By similarity).

See full target information TGFBI

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JACC. Basic to translational science 9:100-116 PubMed38362348

2024

Endothelial Overexpression of TGF-β-Induced Protein Impairs Venous Thrombus Resolution: Possible Role in CTEPH.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena L Bochenek,Kathrin Saar,Maliheh Nazari-Jahantigh,Rajinikanth Gogiraju,Christoph B Wiedenroth,Thomas Münzel,Eckhard Mayer,Ludger Fink,Andreas Schober,Norbert Hübner,Stefan Guth,Stavros Konstantinides,Katrin Schäfer

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2200546 PubMed35901491

2022

Succinylation Inhibits the Enzymatic Hydrolysis of the Extracellular Matrix Protein Fibrillin 1 and Promotes Gastric Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyun Wang,Xiao Shi,Hongcheng Lu,Chen Zhang,Xiang Li,Tiancheng Zhang,Jiajia Shen,Jianfei Wen

PloS one 10:e0144703 PubMed26671068

2015

SLC26A Gene Family Participate in pH Regulation during Enamel Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaifeng Yin,Yuejuan Lei,Xin Wen,Rodrigo S Lacruz,Manoocher Soleimani,Ira Kurtz,Malcolm L Snead,Shane N White,Michael L Paine

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TGFBI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody (AB99562)

Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody (AB99562)

Western blot - Anti-TGFBI antibody (AB99562)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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