Anti-TGFBI antibody [EPR12078(B)] (ab170874) is a rabbit monoclonal antibody that is used to detect TGFBI in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 30 publications
Images
![Western blot - Anti-TGFBI antibody [EPR12078(B)] (AB170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--western-blot-img61087.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TGFBI antibody [EPR12078(B)] (AB170874)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (AB170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128811.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (AB170874)")
![Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (AB170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--immunocytochemistry-immunofluorescence-img1205.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (AB170874)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (AB170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12509.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (AB170874)")
Publications
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- iScience 26:1077692023Spatially distinct otic mesenchyme cells show molecular and functional heterogeneity patterns before hearing onset.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin P Rose,Gabriella Manilla,Beatrice Milon,Ori Zalzman,Yang Song,Thomas M Coate,Ronna HertzanoPubMed 37720106
- Experimental & molecular medicine 55:1713-17192023Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Ram Lee,Seon-Yeong Lee,Jeong Won Choi,In Gyu Um,Hyun Sik Na,Jung Ho Lee,Mi-La ChoPubMed 37524866
- International journal of molecular sciences 24:2023Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Krzistetzko,Cyrill Géraud,Christof Dormann,Anna Riedel,Thomas LeibingPubMed 37446152
- NPJ precision oncology 7:502023High-throughput proteomics profiling-derived signature associated with chemotherapy response and survival for stage II/III colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Biao Ye,Yi-Kan Cheng,Pei-Si Li,Lin Zhang,Lian-Hai Zhang,Yan Huang,Ping Chen,Yi Wang,Chao Wang,Jian-Hong Peng,Li-Shuo Shi,Li Ling,Xiao-Jian Wu,Jun Qin,Zi-Huan Yang,Ping LanPubMed 37258779
- Frontiers in immunology 14:9782362023's protective role in prostate cancer is partly due to the modulation of immune-related pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZainab Al Shareef,Mahmood Y Hachim,Iman M Talaat,Poorna Manasa Bhamidimarri,Mai Nidal Asad Ershaid,Burcu Yener Ilce,Thenmozhi Venkatachalam,Abdulla Eltayeb,Rifat Hamoudi,Ibrahim Y HachimPubMed 36845147
- Cell discovery 8:942022Single-cell transcriptome reveals cellular hierarchies and guides p-EMT-targeted trial in skull base chordoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQilin Zhang,Lijiang Fei,Rui Han,Ruofan Huang,Yongfei Wang,Hong Chen,Boyuan Yao,Nidan Qiao,Zhe Wang,Zengyi Ma,Zhao Ye,Yichao Zhang,Weiwei Wang,Ye Wang,Lin Kong,Xuefei Shou,Xiaoyun Cao,Xiang Zhou,Ming Shen,Haixia Cheng,Zhenwei Yao,Chao Zhang,Guoji Guo,Yao ZhaoPubMed 36127333
- Clinical and translational medicine 12:e7582022TGF-β1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung Eun Baik,Hyeung Ju Park,Raghu P Kataru,Ira L Savetsky,Catherine L Ly,Jinyeon Shin,Elizabeth M Encarnacion,Michele R Cavali,Mark G Klang,Elyn Riedel,Michelle Coriddi,Joseph H Dayan,Babak J MehraraPubMed 35652284
- Tissue engineering and regenerative medicine 19:577-5882022IL-33 Promotes ST2-Dependent Fibroblast Maturation via P38 and TGF-β in a Mouse Model of Epidural Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaoran Wang,Tao Wu,Feng Hua,Jinpeng Sun,Yunfeng Bai,Weishun Wang,Jun Liu,Mingshun ZhangPubMed 35195855
- Canadian journal of gastroenterology & hepatology 2022:72347042022MiR-766-3p Suppresses Malignant Behaviors and Stimulates Apoptosis of Colon Cancer Cells via Targeting TGFBI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianchao Gao,Lexue Fei,Xiaotang Wu,Hua LiPubMed 35083181
- International journal of molecular sciences 23:2022Niclosamide Suppresses Migration and Invasion of Human Osteosarcoma Cells by Repressing TGFBI Expression via the ERK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang-Tsai Yeh,Chiao-Wen Lin,Ko-Hsiu Lu,Yi-Hsien Hsieh,Chao-Bin Yeh,Shun-Fa Yang,Jia-Sin YangPubMed 35008910
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Expected | Predicted |
| Rat | Predicted | Not recommended | Expected | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity).
Alternative names
BIGH3, TGFBI, Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP
Recommended products
Anti-TGFBI antibody [EPR12078(B)] (ab170874) is a rabbit monoclonal antibody that is used to detect TGFBI in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 30 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12078(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TGFBI antibody [EPR12078(B)] (ab170874) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of TGFBI?
Anti-TGFBI [EPR12078(B)] (ab170874) specifically detects a band for TGFBI (UniProt: Q15582) at a molecular weight of 75kDa.
Trusted by the scientific community
Anti-TGFBI [EPR12078(B)] (ab170874) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-TGFBI [EPR12078(B)] (ab170874) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR12078(B)] also available for your convenience:
ab170874, Carrier free - Anti-TGFBI antibody [EPR12078(B)] - BSA and Azide free ab240162
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--western-blot-img61087.jpg "Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)")
Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
TGFBI Western blot staining using rabbit Anti-TGFBI antibody
All lanes: Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874) at 1/1000 dilution
Lane 1: Human fetal kidney lysate at 10 µg
Lane 2: Human fetal liver lysate at 10 µg
SecondaryAll lanes: HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128811.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TGFBI using ab170874 at a 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--immunocytochemistry-immunofluorescence-img1205.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)")
Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
Immunofluorescent staining of Y79 cells labeling TGFBI using ab170874 at a 1/50 dilution (green) and DAPI nuclear staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874), expandable thumbnail](https://content.abcam.com/products/images/tgfbi-antibody-epr12078b-ab170874--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12509.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI using ab170874 at a 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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