AB169771

Anti-TGFBI antibody [EPR12079(B)]

Name: Anti-TGFBI antibody [EPR12079(B)] (ab169771) | Abcam
Brand: Abcam Limited
SKU: AB169771
Price: 4865 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal TGFBI antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

BIGH3, TGFBI, Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI with ab169771 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling TGFBI with ab169771 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

All lanes:

Western blot - Anti-TGFBI antibody [EPR12079(B)] (ab169771) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human small intestine lysate at 10 µg

Lane 3:

Mouse eyeball lysate at 10 µg

Lane 4:

Rat small intestine lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 75 kDa

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CiteAb

Western blot - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

TGFBI western blot using anti-TGFBI antibody [EPR12079(B)] ab169771. Publication image and figure legend from Lv, J., Wang, Z., et al., 2017, J Diabetes Res, PubMed 28713834.

ab169771 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab169771 please see the product overview.

SYFSF inhibited renal fibrosis in DKD rats. (a–d) Immunohistochemical staining of TGF-β1, collagen IV, and fibronectin is shown. (e, f) Western blot and quantitative analysis of TGF-β1 in the cortex. SYFSF and irbesartan inhibited the expression of TGF-β1 in DKD rats. DKD + S represents the DKD rats treated with SYFSF. DKD + I represents the DKD rats treated with irbesartan. *p < 0.05, **p < 0.01, and ***p < 0.001.

false

Carrier free

Anti-TGFBI antibody [EPR12079(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12079(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in cell adhesion (PubMed : 8024701). May play a role in cell-collagen interactions (By similarity).

See full target information TGFBI

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Iranian journal of public health 53:2260-2270 PubMed39544858

2024

Promotes Osteogenic Differentiation and Calcification of Valvular Interstitial Cells by Targeting in Calcific Aortic Valve Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Gu,Rongjin Chen,Jianxiang Song,Zhan Shi,Jixiang Wu,Huiwen Chang,Conghu Yuan,Woda Shi,Yajun Zhang

Biomedicines 12: PubMed38790949

2024

Endurance Training Provokes Arrhythmogenic Right Ventricular Cardiomyopathy Phenotype in Heterozygous Desmoglein-2 Mutants: Alleviation by Preload Reduction.

Applications

Unspecified application

Species

Unspecified reactive species

Larissa Fabritz,Lisa Fortmueller,Katja Gehmlich,Sebastian Kant,Marcel Kemper,Dana Kucerova,Fahima Syeda,Cornelius Faber,Rudolf E Leube,Paulus Kirchhof,Claudia A Krusche

Investigative ophthalmology & visual science 65:21 PubMed38190126

2024

Exploring Corneal Neovascularization: An Integrated Approach Using Transcriptomics and Proteomics in an Alkali Burn Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Manli Deng,Min Li,Lin Liu,Jun Zou,Yiyong Qian

Frontiers in immunology 11:581517 PubMed33414782

2020

-Induced Downregulation of lncRNA Gm28309 Triggers Macrophages Inflammatory Response Through the miR-3068-5p/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingmei Deng,Jia Guo,Zhihua Sun,Laizhen Liu,Tianyi Zhao,Jia Li,Guochao Tang,Hai Zhang,Wenjing Wang,Shuzhu Cao,Dexin Zhu,Tingting Tao,Gang Cao,P I Baryshnikov,Chuangfu Chen,Zongsheng Zhao,Lihua Chen,Hui Zhang

Scientific reports 9:18100 PubMed31792315

2019

CXCR7 promotes migration and invasion in head and neck squamous cell carcinoma by upregulating TGF-β1/Smad2/3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nayoung Kim,Hyewon Ryu,Solbi Kim,Mina Joo,Heung Jin Jeon,Myung-Won Lee,Ik-Chan Song,Mi-Na Kim,Jin-Man Kim,Hyo Jin Lee

Journal of diabetes research 2017:4319057 PubMed28713834

2017

Renoprotective Effect of the Shen-Yan-Fang-Shuai Formula by Inhibiting TNF-/NF-B Signaling Pathway in Diabetic Rats.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Jie Lv,Zhen Wang,Ying Wang,Weiwei Sun,Jingwei Zhou,Mengdi Wang,Wei Jing Liu,Yaoxian Wang

The British journal of dermatology 175:302-13 PubMed26972571

2016

Microarray study reveals a transforming growth factor-β-dependent mechanism of fibrosis in discoid lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

C Solé,M Gimenez-Barcons,B Ferrer,J Ordi-Ros,J Cortés-Hernández

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TGFBI antibody [EPR12079(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

Western blot - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

Western blot - Anti-TGFBI antibody [EPR12079(B)] (AB169771)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (7)

Product promise