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Rabbit Recombinant Monoclonal TGFBI antibody. Suitable for IP, WB and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-TGFBI antibody [EPR17990-13] (AB187085), expandable thumbnail
  • Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (AB187085), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity).

Alternative names

Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Tgfbi

Recommended products

Rabbit Recombinant Monoclonal TGFBI antibody. Suitable for IP, WB and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17990-13
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-TGFBI antibody [EPR17990-13] (ab187085), expandable thumbnail

    Western blot - Anti-TGFBI antibody [EPR17990-13] (ab187085)

    Exposure time : Lane 1: 3 minutes; Lane 2: 1 second; Lanes 3 and 4: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19478074).

    All lanes: Western blot - Anti-TGFBI antibody [EPR17990-13] (ab187085) at 1/1000 dilution

    Lane 1: Rat eyeball lysate at 20 µg

    Lane 2: Mouse eyeball lysate at 20 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Rat liver lysate at 10 µg

    Secondary

    Lanes 1, 3 and 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 75 kDa

    Observed band size: 68 kDa

  • Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (ab187085), expandable thumbnail

    Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (ab187085)

    TGFBI was immunoprecipitated from 0.35 mg of mouse eyeball lysate with ab187085 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187085 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse eyeball lysate 10 μg (Input).

    Lane 2: ab187085 IP in mouse eyeball lysate (+).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab187085 in mouse eyeball lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (ab187085)

    Predicted band size: 75 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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