AB220965

Anti-TGIF antibody

Name: Anti-TGIF antibody (ab220965) | Abcam
Brand: Abcam Limited
SKU: AB220965
Price: 645 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal TGIF antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TGIF1 aa 250-400.

View Alternative Names

TGIF, TGIF1, Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TGIF antibody (AB220965)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TGIF antibody (AB220965)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A549 cells labeling TGIF with ab220965 at 4 μg/ml. Antibody staining is shown in green.

Supplier Data

Western blot - Anti-TGIF antibody (AB220965)

All lanes:

Western blot - Anti-TGIF antibody (ab220965) at 1/100 dilution

All lanes:

RT4 cell extract

Predicted band size: 43 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TGIF1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q15583

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.

See full target information TGIF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 15:555-570 PubMed35611367

2022

is Highly Expressed in Pan-Cancer Samples and Can Promote the Proliferation, Invasion and Migration of Hepatocellular Carcinoma Cells by Activating Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wangxia Tong,Lilan Yang,Li Liu,Xudong Liu,Ning Luo

International journal of molecular medicine 47: PubMed33693954

2021

TGIF1 plays a carcinogenic role in esophageal squamous cell carcinoma through the Wnt/β‑catenin and Akt/mTOR signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Kong,Yang Yu,Hui Guan,Liyang Jiang,Fenghao Sun,Xiaolin Li,Wei Huang,Baosheng Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TGIF antibody

Immunocytochemistry/ Immunofluorescence - Anti-TGIF antibody (AB220965)

Western blot - Anti-TGIF antibody (AB220965)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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